Membrane-Labeled MDCK Cells and Confocal Microscopy for the Analyses of Cellular Volume and Morphology(Molecular and Cell Biology)
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概要
- 論文の詳細を見る
A clone of Madin-Darby canine kidney (MDCK) cells whose cell membrane was stably labeled with expressed cyan fluorescent protein (CFP) was established, and changes in their volume and shape induced by hyposmotic stress were analyzed with confocal microscopy. The membrane-targeted CFP was present not only on the cell membrane but also in the endoplasmic reticulum and Golgi apparatus, but was excluded from the mitochondria and cell nucleus. During hyposmosis, the initial swelling and the following regulatory volume decrease could be accurately measured by summation of the cellular volume in every confocal slice crossing the cell at different heights. Changes in the cellular height roughly paralleled the changes in cellular volume when the mean value was compared, but dissociation as much as 30% was observed for individual cells due to changes in cell shape. The present experimental system, which enables direct measurement of cell volume and simultaneous observation of various intracellular phenomena, would be useful for further investigation of cellular volume regulation.
- 社団法人日本薬学会の論文
- 2008-04-01
著者
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Iida-tanaka Naoko
Department Of Food Science Otsuma Women's University
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KAWANISHI Toru
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences
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TANAKA Hikaru
Department of Pharmacology, Toho University School of
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Tanaka Hikaru
Dep. Of Pharmacology Toho Univ. Fac. Of Pharmaceutical Sciences Jpn
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Tanaka H
Department Of Pharmacology Toho University School Of Pharmaceutical Sciences
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Kawanishi T
Division Of Drugs National Institute Of Health Science
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Kawanishi Toru
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences
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Namekata Iyuki
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Toho University
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TAMURA Miku
Department of Pharmacology, Toho University Faculty of Pharmaceutical Sciences
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KAWAMATA Yuko
Department of Food Science, Otsuma Women's University
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Tanaka Hikaru
Dep. Of Pharmacology Fac. Of Pharmaceutical Sciences Toho Univ.
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Tanaka Hikaru
Department Of Pharmacology Toho University Faculty Of Pharmaceutical Sciences
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KOBAYASI TADASHI
Kobe Pharmaceutical University
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Kawanishi Toru
Division Of Drugs National Institute Of Health Science
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Kawanishi Toru
国立医薬品食品衛生研究所
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Tamura Miku
大妻女子大学 食物学科
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Tamura Miku
Department Of Pharmacology Toho University Faculty Of Pharmaceutical Sciences
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Kawamata Yuko
Department Of Food Science Otsuma Women's University
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古林 隆司
宇部興産株式会社研究開発本部宇部研究所医薬研究部
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Tanaka Hikaru
Department Of Internal Medicine Sasebo Senjyu Hospital
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Namekata Iyuki
Department Of Pharmacology Toho University Faculty Of Pharmaceutical Sciences
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Tanaka Hikaru
Department Of Internal Medicine Hirosaki Municipal Hospital
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Namekata Iyuki
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Toho University
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Tanaka Hikaru
Department of Endocrinology and Metabolism, Hirosaki University
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Iida-Tanaka Naoko
Department of Biochemistry, Teikyo University School of Medicine
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