Phospholipase C Inhibitors Suppress Spontaneous Mechanical Activity of Guinea Pig Urinary Bladder Smooth Muscle(Pharmacology)
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概要
- 論文の詳細を見る
Urinary bladder smooth muscle (UBSM) exhibits spontaneous rhythmic contraction. This spontaneous mechanical activity is generated in the presence of neuronal blockade and thus is myogenic in origin. The spontaneous myogenic contraction of UBSM may be the fundamental determinant of the physiological functions of the urinary bladder to store and excrete urine. Although the mechanisms by which UBSM generates spontaneous contraction have not been completely ascertained, its induction has been suggested to be intimately associated with smooth muscle cell action potentials to enhance extracellular Ca^<2+> influx through voltage-gated L-type Ca^<2+> channels. However, the alteration of membrane electrical activity does not seem to be the exclusive trigger mechanism for the generation of the spontaneous contraction. In the present study, we show that spontaneous mechanical activity of guinea pig UBSM is substantially diminished by an inhibitor of phospholipase C (PLC), U-73122, but is not affected by its inactive form, U-73343. Significant attenuation of the mechanical activity can be also obtained with another PLC inhibitor 2-nitro-4-carboxyphenyl-N,N-diphenylcarbamate. Our present findings suggest a significant role for the activation of PLC and subsequent inositol 1,4,5-trisphosphate-induced Ca^<2+> release mechanism as an alternative triggering system for inducing spontaneous mechanical activity of UBSM. The present results support the idea that the action potential is not the sole pacemaker mechanism by which spontaneous contraction is induced in UBSM.
- 公益社団法人日本薬学会の論文
- 2003-08-01
著者
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KOIKE Katsuo
Department of Chemical Pharmacology, Toho University School of Pharmaceutical Sciences
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TANAKA Hikaru
Department of Pharmacology, Toho University School of
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SHIGENOBU Koki
Department of Pharmacology, Toho University School of
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Koike Katsuo
東京薬科大学 内分泌分子薬理学
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Koike K
Toho Univ. School Of Pharmaceutical Sci. Chiba Jpn
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Koike Katsuo
Department Of Chemical Pharmacology Toho University Faculty Of Pharmaceutical Sciences
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Tanaka Hikaru
Dep. Of Pharmacology Toho Univ. Fac. Of Pharmaceutical Sciences Jpn
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Shigenobu K
Department Of Pharmacology Toho University School Of Pharmaceutical Sciences
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Shigenobu Koki
Department Of Chemical Pharmacology Faculty Of Pharmaceutical Sciences The University Of Tokyo
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Tsuneoka Yayoi
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Toho University
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Tanaka Yoshio
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Toho University
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Tanaka Hikaru
Department Of Pharmacology Toho University Faculty Of Pharmaceutical Sciences
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KOIKE Katsuo
Toho University School of Pharmaceutical Sciences
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Koike K
Toho Univ. Chiba Jpn
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Tanaka Yoshio
Dep. Of Chemical Pharmacology Fac. Of Pharmaceutical Sciences Toho Univ.
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Toyoda Yoshihiro
Sagami Research Laboratory Wakamoto Pharmaceutical Co. Ltd.
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Horinouchi Takahiro
Department Of Chemical Pharmacology Toho University School Of Pharmaceutical Sciences
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Horinouchi T
Department Of Chemical Pharmacology Toho University School Of Pharmaceutical Sciences
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OKAMOTO Takao
Department of Chemical Pharmacology, Toho University School of Pharmaceutical Sciences
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Okamoto Takao
Department Of Chemical Pharmacology Toho University School Of Pharmaceutical Sciences
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Tanaka Yoshio
Department Of Chemical Pharmacology Toho University Faculty Of Pharmaceutical Sciences
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Tanaka Yoshio
Department Of Pharmacology Toho University Faculty Of Pharmaceutical Sciences
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IMAI Toshiyasu
Department of Pharmacology, Toho University School of Pharmaceutical Sciences
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Tanaka Hikaru
Department Of Internal Medicine Sasebo Senjyu Hospital
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Imai Toshiyasu
Department Of Pharmacology Toho University School Of Pharmaceutical Sciences
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Horinouchi Takahiro
Department Of Cellular Pharmacology Hokkaido University Graduate School Of Medicine
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Tanaka Yoshio
Department Of Applied Chemistry Faculty Of Engineering Yokohama National University
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Horinouchi Takahiro
Department Of Cellular Pharmacology Graduate School Of Medicine Hokkaido University
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Tanaka Hikaru
Department Of Internal Medicine Hirosaki Municipal Hospital
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Tanaka Hikaru
Department of Endocrinology and Metabolism, Hirosaki University
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