β_1-Adrenoceptor-mediated relaxation with isoprenaline and the role of MaxiK channels in guinea-pig esophageal smooth muscle

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The possible functional coupling between β_1-adrenoceptor and MaxiK channels which results in smooth muscle relaxation was examined in the guinea-pig esophageal muscularis mucosae. Isoprenaline-elicited relaxation of esophageal smooth muscle was confirmed to be mediated through β_1-adrenoceptors as the response was competitively antagonized by a β_1-selective antagonist atenolol with a pA_2 value of 7.01. Iberiotoxin (IbTx, 10^<-7> M), a selective MaxiK channel inhibitor, substantially diminished the relaxant response to isoprenaline. The extent of the MaxiK channel contribution to the relaxant response was 15-40% of the control response when estimated as the E_<50%>-E_<max> responses to isoprenaline. The relaxation to isoprenaline was also attenuated by high-KCl (80 mM) to the same degree as the relaxant response generated in the presence of IbTx, and thus the estimated extent of the K^+ channel contribution was 10-40%. These findings indicate that β_1-adrenoceptors are substantially coupled with MaxiK channels to produce relaxation of esophageal smooth muscle in the guinea-pig. Although MaxiK channels account for the contribution of K^+ channels to the β_1-adrenoceptor-mediated relaxation in this smooth muscle preparation, their contribution seems to be less when compared to the β_1-adrenoceptor-mediated relaxation of tracheal smooth muscle.
日本平滑筋学会の論文