Differential Coupling of Human Endothelin Type A Receptor to Gq/11 and G12 Proteins: the Functional Significance of Receptor Expression Level in Generating Multiple Receptor Signaling
スポンサーリンク
概要
- 論文の詳細を見る
This study examines the influence of receptor expression level on signaling pathways activated via endothelin type A receptor (ETAR) expressed in Chinese hamster ovary cells at 32,100 (ETAR-high-CHO) and 893 (ETAR-low-CHO) fmol · mg protein−1. Endothelin-1 (ET-1) elicited a sustained increase in intracellular Ca2+ concentration ([Ca2+]i), which was dependent on Gq/11 protein, phospholipase C (PLC), Na+/H+ exchanger (NHE), and p38 mitogen–activated protein kinase (p38MAPK) in ETAR-high-CHO, whereas the sustained [Ca2+]i increase was negligible in ETAR-low-CHO. Functional study with CytosensorTM microphysiometer showed that ET-1 evoked an NHE1-mediated increase in extracellular acidification rate (ECAR) in ETAR-high-CHO and ETAR-low-CHO. In ETAR-high-CHO, the ECAR response at 30 min after ET-1 stimulation was insensitive to Gq/11 and PLC inhibitors, but sensitive to the p38MAPK inhibitor. In ETAR-low-CHO, the ECAR response at 30 min was sensitive to these inhibitors. Western blot analysis demonstrated that ET-1–induced p38MAPK phosphorylation in ETAR-low-CHO but not in ETAR-high-CHO was mediated via Gq/11 and PLC. The Gq/11/PLC-independent p38MAPK phosphorylation in ETAR-high-CHO was suppressed by expression of the C terminus of Gα12 protein to disrupt receptor-G12 protein coupling. These results provide evidence for multiple signaling pathways of ETAR that were activated via at least the Gq/11/PLC/NHE, G12/p38MAPK/NHE, and Gq/11/PLC/p38MAPK/NHE cascades in an expression level–dependent manner.
- 社団法人 日本薬理学会の論文
著者
-
Miwa Soichi
Department Of Cellular Pharmacology Hokkaido University Graduate School Of Medicine
-
MURAMATSU Ikunobu
Division of Pharmacology, Department of Biochemistry and Bioinformative Sciences, School of Medicine
-
Nishiya Tadashi
Department Of Pharmacology Graduate School Of Pharmaceutical Sciences Hokkaido University
-
Horinouchi Takahiro
Department Of Chemical Pharmacology Toho University School Of Pharmaceutical Sciences
-
Horinouchi Takahiro
北海道大学 医学研究科細胞薬理学分野
-
Muramatsu Ikunobu
Division Of Pharmacology Department Of Biochemistry And Bioinformative Sciences School Of Medicine U
-
ASANO Hiroshi
Department of Neurosurgery,Nihon Universtity School of Medicine
-
Nishimoto Arata
Department of Cellular Pharmacology, Hokkaido University Graduate School of Medicine, Japan
-
HIGA Tunaki
Department of Cellular Pharmacology, Hokkaido University Graduate School of Medicine
-
Horinouchi Takahiro
Department Of Cellular Pharmacology Hokkaido University Graduate School Of Medicine
-
Nishimoto Arata
Department Of Cellular Pharmacology Hokkaido University Graduate School Of Medicine
-
Higa Tunaki
Department Of Cellular Pharmacology Hokkaido University Graduate School Of Medicine
-
Asano Hiroshi
Department Of Cellular Pharmacology Hokkaido University Graduate School Of Medicine
-
Nishiya Tadashi
Department Of Cellular Pharmacology Hokkaido University Graduate School Of Medicine
-
Miwa Soichi
Department Of Cellular Pharmacology Graduate School Of Medicine Hokkaido University
-
Asano Hiroshi
Department Of Cellular Pharmacology Graduate School Of Medicine Hokkaido University
-
Horinouchi Takahiro
Department Of Cellular Pharmacology Graduate School Of Medicine Hokkaido University
-
Nishiya Tadashi
Department Of Cellular Pharmacology Graduate School Of Medicine Hokkaido University
-
Muramatsu Ikunobu
Division of Pharmacology, Department of Biochemistry and Bioinformative Sciences, School of Medicine, University of Fukui, Japan
-
Higa Tunaki
Department of Cellular Pharmacology, Hokkaido University Graduate School of Medicine, Japan
関連論文
- Jab1 regulates levels of endothelin type A and B receptors by promoting ubiquitination and degradation
- Identification of Cysteine-Rich Epidermal Growth Factor–Like Domain 1α (CRELD1α) as a Novel α1A-Adrenoceptor–Down-Regulating Protein and Establishment of an α1L-Adrenoceptor–Expressing Cell Line
- Improvement of Postischemic Dopaminergic Dysfunction by Edaravone, a Free Radical Scavenger
- Mutational Analysis of the α_-Adrenergic Receptor Binding Pocket of Antagonists by Radioligand Binding Assay(Pharmacology)
- Beta-Blockers Show Inverse Agonism to a Novel Constitutively Active Mutant of β_1-Adrenoceptor
- Pharmacological and Molecular Characterization of Novel SWR-Compounds for β-Adrenergic Receptors as assessed by Molecular Modeling, Site-Directed Mutagenesis, Binding Affinity and Functional Activity
- Quantifying Receptor Properties : the Tissue Segment Binding Method-a Powerful Tool for the Pharmacome Analysis of Native Receptors
- Endothelin-1 decreases [Ca2+]i via Na+/Ca2+ exchanger in CHO cells stably expressing endothelin ETA receptor.
- Evaluation of β_-Adrenoceptors in Rabbit Heart by Tissue Segment Binding Assay
- Effect of Cigarette Smoke Components on Vascular Smooth Muscle Cell Migration Toward Platelet-Derived Growth Factor BB
- Assessment of Muscarinic Receptor Subtypes in Human and Rat Lower Urinary Tract by Tissue Segment Binding Assay
- Ca^ entry channels involved in endothelin-1-induced contractions of vascular smooth muscle cells
- Ca^ Entry Channels in Rat Thoracic Aortic Smooth Muscle Cells Activated by Endothelin-1
- The β_2- and β_3-Adrenoceptor-Mediated Relaxation Induced by Isoprenaline and Salbutamol in Guinea Pig Taenia Caecum
- Signal Transduction Pathway Involved in β_3 - Adrenoceptor - Mediated Relaxation in Guinea Pig Taenia Caecum
- 109. Positive Contrast Cerebral Vintriculography Using Water-Soluble Media
- 128 128. Histological Study of the Brain Stem in Cases of Supratentorial Tumors
- 45 Studies on Hemodynamic Changes Following the Head Injury
- Nitric Oxide (NO) Primarily Accounts for Endothelium-Dependent Component of β-Adrenoceptor-Activated Smooth Muscle Relaxation of Mouse Aorta in Response to Isoprenaline
- (+__-)-Pindolol Acts as a Partial Agonist at Atypical β-Adrenoceptors in the Guinea Pig Duodenum
- Endothelin Type B Receptor–Induced Sustained Ca2+ Influx Involves Gq/11/Phospholipase C–Independent, p38 Mitogen-Activated Protein Kinase–Dependent Activation of Na+/H+ Exchanger
- Differential Coupling of Human Endothelin Type A Receptor to G_ and G_ Proteins : the Functional Significance of Receptor Expression Level in Generating Multiple Receptor Signaling
- Differential Coupling of Human Endothelin Type A Receptor to Gq/11 and G12 Proteins: the Functional Significance of Receptor Expression Level in Generating Multiple Receptor Signaling
- Functional Role of Na^+/H^+ Exchanger in Ca^ Influx Mediated via Human Endothelin Type A Receptor Stably Expressed in Chinese Hamster Ovary Cells
- Characterization of Noradrenaline-Induced Increases in Intracellular Ca^ Levels in Chinese Hamster Ovary Cells Stably Expressing Human α_-Adrenoceptor
- OCTN2VT, a splice variant of OCTN2, does not transport carnitine because of the retention in the endoplasmic reticulum caused by insertion of 24 amino acids in the first extracellular loop of OCTN2
- Further Evidence that (±)-Carteolol-induced Relaxation is Mediated by β_2-Adrenoceptors but not by β_3-Adrenoceptors in the Guinea Pig Taenia Caecum
- α_1-Adrenoceptor pharmacome : α_-Adrenoceptor and α_-adrenoceptor in the lower urinary tract
- β_1-Adrenoceptor-mediated relaxation with isoprenaline and the role of MaxiK channels in guinea-pig esophageal smooth muscle
- MaxiK Channel Mediates β_2-Adrenoceptor-activated Relaxation to Isoprenaline through cAMP-dependent and -independent Mechanisms in Guinea-pig Tracheal Smooth Muscle
- The β_3-Adrenoceptor-Mediated Relaxation Induced by Dopamine in Guinea Pig Taenia Caecum
- Correlation between Coronary Arterial Remodeling and the Ability of Insulin Secretion in Nondiabetic Patients
- Cloning and functional characterization of a novel up-regulator, cartregulin, of carnitine transporter, OCTN2
- Studies on Trapping of Laser-Produced Plasma by SL-1 Surmac
- Functional Contribution of Voltage-Dependent and Ca^2+-Activated K^+ (BK_ca) Channels to the Relaxation of Guinea-Pig Aorta in Response to Natriuretic Peptides
- Structure-Activity Relationship Studies of (±)- Terbutaline and (±)-Fenoterol on β_3-Adrenoceptors in the Guinea Pig Gastric Fundus
- Adrenaline produces the relaxation of guinea-pig airway smooth muscle primarily through the mediation of β_2-adrenoceptors
- Phospholipase C Inhibitors Suppress Spontaneous Mechanical Activity of Guinea Pig Urinary Bladder Smooth Muscle(Pharmacology)
- The β_2- and β_3-Adrenoceptor-Mediated Relaxation Induced by Fenoterol in Guinea Pig Taenia Caecum
- Identification of Cysteine-Rich Epidermal Growth Factor-Like Domain lα(CRELD1α) as a Novel α_ -Adrenoceptor-Down-Regulating Protein and Establishment of an α_-Adrenoceptor-Expressing Cell Line
- Assessment of Novel Muscarinic Acetylcholine Receptors in Rat Cerebral Cortex by a Tissue Segment Binding Method
- ECHOCARDIOGRAPHIC ASSESSMENT OF ATRIAL TRANSPORT FUNCTION IN SICK SINUS SYNDROME : Arrhythmia : FREE COMMUNICATIONS (Abstract) : 45 Annual Scientific Meeting, Japanese Circulation Society
- STUDIES OF ATRIAL VASODEPRESSOR REFLEX IN PATIENTS WITH SICK SINUS SYNDROME : Arrhythmia I, Immunity & Heredity, Contractile Protein : IVth Auditorium : Proceedings of the 43rd Annual Meeting of the Japanese Circulation Society, Tokyo, 1979
- -159-SINUS NODE FUNCTION IN CAROTID SINUS HYPERSENSITIVITY (2) : Arrythmia, His Bundle Electrogram , Electrophysiology : PROCEEDINGS OF THE 42nd ANNUAL MEETING OF THE JAPANESE CIRCULATION SOCIETY
- The β_3-Adrenoceptor-Mediated Relaxation Induced by Epinephrine in Guinea Pig Taenia Caecum
- Partial Agonistic Effects of Carteolol on Atypical β-Adrenoceptors in the Guinea Pig Gastric Fundus
- Involvement of Polyubiquitin Chains via Specific Chain Linkages in Stress Response in Mammalian Cells(Biochemistry)
- Vapor Etching of Boron Monophosphide by Gaseous Hydrogen Chloride
- Endothelin Type B Receptor–Induced Sustained Ca2+ Influx Involves Gq/11/Phospholipase C–Independent, p38 Mitogen-Activated Protein Kinase–Dependent Activation of Na+/H+ Exchanger
- Na, K-, Mg- AND HCO3-ADENOSINE TRIPHOSPHATASES IN THE RABBIT BRAIN CHOROID PLEXUS
- Characterization of Noradrenaline-Induced Increases in Intracellular Ca2+ Levels in Chinese Hamster Ovary Cells Stably Expressing Human α1A-Adrenoceptor
- Effect of Cigarette Smoke Components on Vascular Smooth Muscle Cell Migration Toward Platelet-Derived Growth Factor BB
- Endothelin Type B Receptor-Induced Sustained Ca^ Influx Involve G_/Phospholipase C-Independent, p38 Mitogen-Activated Protein Kinase-Dependent Activation of Na^+/H^+ Exchanger
- Function and Regulation of Endothelin Type A Receptor-Operated Transient Receptor Potential Canonical Channels
- Nicotine- and Tar-Free Cigarette Smoke Induces Cell Damage Through Reactive Oxygen Species Newly Generated by PKC-Dependent Activation of NADPH Oxidase
- Nicotine- and Tar-free Cigarette Smoke Extract Induces Cell Injury via Intracellular Ca2+-Dependent Subtype-Specific Protein Kinase C Activation
- Beta-Blockers Show Inverse Agonism to a Novel Constitutively Active Mutant of .BETA.1-Adrenoceptor
- 109. Positive Contrast Cerebral Vintriculography Using Water-Soluble Media
- Functional Role of Na+/H+ Exchanger in Ca2+ Influx Mediated via Human Endothelin Type A Receptor Stably Expressed in Chinese Hamster Ovary Cells
- Endothelin Receptor Signaling: New Insight Into Its Regulatory Mechanisms
- Involvement of .BETA.3-Adrenoceptor in the Relaxation Response in Guinea Pig Taenia Caecum.
- Nicotine- and Tar-free Cigarette Smoke Extract Induces Cell Injury via Intracellular Ca^-Dependent Subtype-Specific Protein Kinase C Activation
- M1 is a major subtype of muscarinic acetylcholine receptors on mouse colonic epithelial cells