Quantifying Receptor Properties : the Tissue Segment Binding Method-a Powerful Tool for the Pharmacome Analysis of Native Receptors
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概要
- 論文の詳細を見る
The radioligand binding assay technique is an extremely powerful tool for studying receptors. It allows an analysis of the interactions of hormones, neurotransmitters, and related drugs with their receptors. Most of the binding assays have widely been applied to crude membrane fractions prepared from many tissues, but in the conventional method, there are some limitations such as a yield loss of receptor-bearing membranes and a change in receptor environment upon homogenization and fractionation. Recently, in order to overcome these problems, a binding assay has been developed using intact tissue segments. This article presents a brief overview of the tissue segment binding assay that has been developed mainly in our department. Practical guidelines for setting up this new assay are presented, including segment preparation, choice of appropriate radioligand, optimizing assay conditions, and appropriate methods for data analysis. The unique advantages and disadvantages of the tissue segment binding method are discussed in comparison with those of conventional membrane binding methods. We suggest that the tissue segment binding method is a powerful tool for detecting the native properties of receptors occurring in tissues and cells without altering their environment.
- 社団法人 日本薬理学会の論文
- 2005-08-20
著者
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Hiraizumi Hiraoka
Division Of Pharmacology Department Of Biochemistry And Bioinformative Sciences School Of Medicine U
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Miwa Soichi
Department Of Cellular Pharmacology Hokkaido University Graduate School Of Medicine
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TANAKA Takashi
Division of Pharmacology, Department of Biochemistry and Bioinformative Sciences, School of Medicine
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MURAMATSU Ikunobu
Division of Pharmacology, Department of Biochemistry and Bioinformative Sciences, School of Medicine
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SUZUKI Fumiko
Division of Pharmacology, Department of Biochemistry and Bioinformative Sciences, School of Medicine
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LI Zhang
Division of Pharmacology, Department of Biochemistry and Bioinformative Sciences, School of Medicine
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ANISUZZAMAN Abu
Division of Pharmacology, Department of Biochemistry and Bioinformative Sciences, School of Medicine
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YAMAMOTO Hatsumi
Division of Pharmacology, Department of Biochemistry and Bioinformative Sciences, School of Medicine
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HORINOUCHI Takahiro
Division of Pharmacology, Department of Biochemistry and Bioinformative Sciences, School of Medicine
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MORISHIMA Shigeru
Division of Pharmacology, Department of Biochemistry and Bioinformative Sciences, School of Medicine
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Anisuzzaman Abu
Div. Of Pharmacology Dep. Of Biochemistry And Bioinformative Sciences School Of Medicine Univ. Of Fu
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Horinouchi Takahiro
北海道大学 医学研究科細胞薬理学分野
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Suzuki Fumiko
Division Of Pharmacology Department Of Biochemistry And Bioinformative Sciences University Of Fukui
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Muramatsu Ikunobu
Organization For Life Sci. Advancement Programs Univ. Of Fukui Jpn
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Muramatsu Ikunobu
Division Of Pharmacology Department Of Biochemistry And Bioinformative Sciences School Of Medicine U
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Muramatsu Ikunobu
Division Of Pharmacology Department Of Biochemistry And Bioinformative Sciences University Of Fukui
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Suzuki Fumio
Division Of Pharmacology Department Of Biochemistry And Bioinformative Sciences University Of Fukui
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Muramatsu I
Division Of Pharmacology Department Of Biochemistry And Bioinformative Sciences University Of Fukui
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Yoshiki Hatsumi
Division Of Pharmacology Department Of Biochemistry And Bioinformative Sciences School Of Medicine U
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Suzuki Fumiko
Division Of Pharmacology Department Of Biochemistry And Bioinformative Sciences School Of Medicine U
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Miwa Soichi
Division Of Pharmacology Department Of Biochemistry And Bioinformative Sciences School Of Medicine U
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Taniguchi T
Division Of Pharmacology Department Of Biochemistry And Bioinformative Sciences University Of Fukui
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Miwa Soichi
Dep. Of Cellular Pharmacology Hokkaido Univ. Graduate School Of Medicine Jpn
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Anisuzzaman Abu
Division Of Pharmacology Department Of Biochemistry And Bioinformative Sciences School Of Medicine U
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Morishima Shigeru
Division Of Pharmacology Department Of Biochemistry And Bioinformative Sciences School Of Medicine U
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Li Zhang
Division Of Pharmacology Department Of Biochemistry And Bioinformative Sciences School Of Medicine U
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Horinouchi Takahiro
Department Of Cellular Pharmacology Hokkaido University Graduate School Of Medicine
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Horinouchi Takahiro
Department Of Cellular Pharmacology Graduate School Of Medicine Hokkaido University
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Tanaka Takashi
Division Of Health Sciences Graduate School Of Medicine Osaka University
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