Functional Role of Na+/H+ Exchanger in Ca2+ Influx Mediated via Human Endothelin Type A Receptor Stably Expressed in Chinese Hamster Ovary Cells

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概要

• 論文の詳細を見る

• This study examines the functional role of Na+/H+ exchanger (NHE) in Ca2+ influx mediated by human endothelin type A receptor (ETAR) expressed in Chinese hamster ovary (CHO) cells. Endothelin-1 (ET-1) increased extracellular acidification rate (ECAR), which was abolished by 5-(N-ethyl-N-isopropyl)amiloride (EIPA), an NHE inhibitor. EIPA and KB-R7943, a Na+/Ca2+ exchanger (NCX) inhibitor, inhibited ET-1-induced sustained increases in intracellular Ca2+ concentration ([Ca2+]i), and EIPA had no effect on [Ca2+]i after KB-R7943 treatment. ET-1-elicited sustained [Ca2+]i increase was suppressed by reducing extracellular Na+ concentration. These results suggest that possible coupling of NHE with NCX via Na+ transport is involved in ETAR-mediated sustained [Ca2+]i increase.

• 公益社団法人 日本薬理学会の論文

著者

• Muramatsu Ikunobu

  Division Of Pharmacology Department Of Biochemistry And Bioinformative Sciences School Of Medicine U

• Nishimoto Arata

  Department of Cellular Pharmacology, Hokkaido University Graduate School of Medicine, Japan

• Miyake Yumie

  Department Of Cellular Pharmacology Hokkaido University Graduate School Of Medicine

• Morishima Shigeru

  Division Of Pharmacology Department Of Biochemistry And Bioinformative Sciences School Of Medicine U

• Nishimoto Arata

  Department Of Cellular Pharmacology Hokkaido University Graduate School Of Medicine

• Miwa Soichi

  Department Of Cellular Pharmacology Graduate School Of Medicine Hokkaido University

• Horinouchi Takahiro

  Department Of Cellular Pharmacology Graduate School Of Medicine Hokkaido University

• Nishiya Tadashi

  Department Of Cellular Pharmacology Graduate School Of Medicine Hokkaido University

• Horinouchi Takahiro

  Department of Cellular Pharmacology, Hokkaido University Graduate School of Medicine, Japan

• Morishima Shigeru

  Division of Pharmacology, Department of Biochemistry and Bioinformative Sciences, School of Medicine, University of Fukui, Japan

• Nishiya Tadashi

  Department of Cellular Pharmacology, Hokkaido University Graduate School of Medicine, Japan

• Miyake Yumie

  Department of Cellular Pharmacology, Hokkaido University Graduate School of Medicine, Japan

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