Nicotine- and Tar-free Cigarette Smoke Extract Induces Cell Injury via Intracellular Ca<SUP>2+</SUP>-Dependent Subtype-Specific Protein Kinase C Activation
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概要
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Nicotine- and tar-free cigarette smoke extract (CSE) is reported to induce cell damage via activation of protein kinase C (PKC) and NADPH oxidase (NOX) in rat C6 glioma cells. Here we determined PKC isozyme(s) activated by CSE and their activation mechanism. In C6 glioma cells, mRNAs for PKC<I>α</I>, PKC<I>δ</I>, PKC<I>ε</I>, and PKC<I>ι</I> were expressed. CSE triggered translocation of PKC<I>α</I> and PKC<I>ε</I> to plasma membrane. CSE-induced cell damage and PKC translocation were inhibited by chelating intracellular Ca<SUP>2+</SUP> but not extracellular Ca<SUP>2+</SUP>. These results suggest that CSE induces cell damage through intracellular Ca<SUP>2+</SUP>-dependent activation of PKC<I>α</I> and PKC<I>ε</I> and subsequent NOX activation.
著者
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Higashi Tsunehito
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Miwa Soichi
Department Of Cellular Pharmacology Graduate School Of Medicine Hokkaido University
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Mai Yosuke
Department Of Cellular Pharmacology Graduate School Of Medicine Hokkaido University
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Horinouchi Takahiro
Department Of Cellular Pharmacology Graduate School Of Medicine Hokkaido University
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Terada Koji
Department Of Cellular Pharmacology Graduate School Of Medicine Hokkaido University
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Harada Takuya
Department of Cellular Pharmacology, Hokkaido University Graduate School of Medicine, Japan
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Horiguchi Mika
Department of Cellular Pharmacology, Hokkaido University Graduate School of Medicine, Japan
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Hatate Chizuru
Department of Cellular Pharmacology, Hokkaido University Graduate School of Medicine, Japan
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Nepal Prabha
Department of Cellular Pharmacology, Hokkaido University Graduate School of Medicine, Japan
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Mai Yosuke
Department of Cellular Pharmacology, Hokkaido University Graduate School of Medicine, Japan
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Miwa Soichi
Department of Cellular Pharmacology, Hokkaido University Graduate School of Medicine, Japan
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Higashi Tsunehito
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science:(Present office)Department of Cellular Pharmacology, Graduate School of Medicine, Hokkaido University
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HORIGUCHI Mika
Department of Cellular Pharmacology, Hokkaido University Graduate School of Medicine
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NEPAL Prabha
Department of Cellular Pharmacology, Hokkaido University Graduate School of Medicine
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HARADA Takuya
Department of Cellular Pharmacology, Hokkaido University Graduate School of Medicine
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HIGASHI Tsunehito
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science
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