Multi-Spectral Two-Photon Excited Fluorescence Microscopy Using Supercontinuum Light Source
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概要
- 論文の詳細を見る
We report on a novel technique of multi-spectral two-photon-excited fluorescence microscopy by use of broadband supercontinuum light. A supercontinuum in the near-infrared region is generated in a 4.5-mm-long photonic crystal fiber using a Ti:sapphire femtosecond oscillator. The supercontinuum is used as a multi-spectral excitation light source in a two-photon excited fluorescence microscope. We demonstrate that three-color fluorescence images of organelles in a cell can be simultaneously acquired.
- INSTITUTE OF PURE AND APPLIED PHYSICSの論文
- 2005-01-10
著者
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Fukui Kiichi
Department Of Biotechnology Faculty Of Engineering Graduate School Of Osaka University
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Watanabe Wataru
Department Of Applied Physics Graduate School Of Engineering Osaka University
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Isobe Keisuke
Department Of Material And Life Science Graduate School Of Engineering Osaka University
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Itoh Kazuyoshi
Department Of Applied Chemistry Faculty Of Engineering & High Technology Research Center Kansai
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Matsunaga Sachihiro
Department Of Applied Biological Science Faculty Of Science And Technology Tokyo University Of Science
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Higashi Tsunehito
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science:(Present office)Department of Cellular Pharmacology, Graduate School of Medicine, Hokkaido University
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Higashi Tsunehito
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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