Nonlinear Phase Imaging Using Two-Beam Interferometry in Stimulated Parametric Emission Microscopy
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概要
- 論文の詳細を見る
We demonstrate that two-beam interferometry is applicable to phase detection of nonlinear susceptibility of biological samples in the stimulated parametric emission (SPE) microscopy, which is one of the coherent nonlinear optical microscopy techniques. We experimentally observed stained mouse kidney cells and successfully confirmed nonlinear phase shift associated with an electronic resonance at stained nuclei. In addition, phase blurring was found to be almost completely eliminated in a nonlinear differential interferometric contrast image, which can be produced through a simple post-processing procedure.
- 2009-06-25
著者
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Fukui Kiichi
Department Of Biotechnology Faculty Of Engineering Graduate School Of Osaka University
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Omura Gen
Department of Biology, Faculty of Science, Kobe University
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Ozeki Yasuyuki
Department Of Biology School Of Education Waseda University
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Itoh Kazuyoshi
Department Of Applied Chemistry Faculty Of Engineering & High Technology Research Center Kansai
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Suzuki Takeshi
Department Of Allergy And Rheumatology Graduate School Of Medicine University Of Tokyo
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Yamagiwa Masatomo
Department of Material and Life Science, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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Itoh Kazuyoshi
Department of Material and Life Science, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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