Cellular Toxicity of Cadmium Ions and Their Detoxification by Heavy Metal-Specific Plant Peptides, Phytochelatins, Expressed in Mammalian Cells.
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概要
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The apoptotic cell death of Jurkat cells due to Cd2+ toxicity was studied by fluorescence microscopic observation and DNA fragmentation assaying. It was suggested that the apoptotic response to Cd2+ was less clear than that to a typical apoptosis inducer, ultraviolet light (254 nm). Examination of MAP kinase phosphorylation (p38, JNKs, and c-Jun) due to Cd2+ toxicity indicated that the phosphorylation was very slowly activated (4h after stimulation), while UV light could activate the phosphorylation immediately. Therefore, it was suggested that Cd2+ may not be a typical apoptosis inducer. Antioxidants [glutathione (GSH) and N-acetylcysteine (NAC)] could detoxify Cd2+, indicating that the toxicity is a kind of oxidative stress. The detoxification effect of antioxidants showed cooperation with Bcl-2, suggesting that Cd2+-treatment causes diversified toxic signals including oxidative stress. On the addition of a plant-specific peptide, phytochelatin [PC7, (γGlu-Cys)7 Gly], to the medium, the detoxification of Cd2+ and cooperation with Bcl-2 were more intense than in the cases of GSH and NAC. Using an appropriate vector, a PC synthase gene was transferred from Arabidopsis thaliana to the Jurkat cell. The transfectant exhibited resistance to Cd2+ and production of plant-specific PC (PC2-6).
- 社団法人 日本生化学会の論文
著者
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Hirata Kazumasa
Department of Applied Environmental Biology, Graduate School of Pharmaceutical Sciences, Osaka Unive
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Mizuno Haruo
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Imanaka Tadayuki
Department Of Applied Biotechnology Faculty Of Engineering Osaka University
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Fukui Kiichi
Department Of Biotechnology Faculty Of Engineering Graduate School Of Osaka University
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Miyamoto Kazuhisa
Department Of Biochemical Engineering Faculty Of Pharmaceutical Sciences Osaka University
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Satofuka Hiroyuki
School Of Materials Science Japan Advanced Institute Of Science And Technology
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Eguchi Yutaka
Department Of Critical And Intensive Care Medicine Shiga University Of Medical Science
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Amano Satoshi
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Takagi Masahiro
School Of Material Science Japan Advanced Institute Of Science And Technology
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Mizuno Haruo
Department of Biotechnology, Graduate School of Engineering, Osaka University
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