Solution properties of phycocyanin. VI. The characterization of the phycocyanin monomer.

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概要

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• The monomer molecular weight of phycocyanin was determined by sedimentation equilibrium measurements in 6 mol/1 guanidine hydrochloride (GndCl) and by sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis. The former measurement gave the monomer molecular weight of 4.0×10<SUP>4</SUP>. From the latter, the phycocyanin was found to be composed of two kinds of subunits, the molecular weights of which were 1.7×10<SUP>4</SUP> and 2.0×10<SUP>4</SUP>. Assumimg that the phycocyanin monomer consists of two polypeptide chains, the molecular weight becomes 3.7×10<SUP>4</SUP>. These values coincide with the value of 4.2×10<SUP>4</SUP> assumed in our previous works, which corresponds to onesixth of the hexamer molecular weight. The sedimentation coefficient, <I>s</I><SUB>25w</SUB>, was 2.21 Svedberg unit, and the intrinsic viscosity in 6 mol/1 GndCl was 19.5 ml/g, at 25.0 °C. From these values, it was clear that the phycocyanin monomer was unfolded in the solvent.

• 公益社団法人 日本化学会の論文

著者

• Mizuno Haruo

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Iso Naomichi

  Department Of Food Science And Technology Tokyo University Of Fisheries

• Matsui Yoshiyuki

  Department Of Urology Kobe City General Hospital

• Saito Takahide

  Department of Chemistry Faculty of Science Tokyo University of Education

• Ozeki Fumiaki

  Department of Food Science and Technology, Tokyo University of Fisheries

• Matsui Yoshiyuki

  Department of Food Science and Technology, Tokyo University of Fisheries

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