Solution properties of phycocyanin. V. Studies of the self-association reaction of phycocyanin in a pH 5.4 solution.
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概要
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The self-association of phycocyanin in a buffer solution of pH 5.4 with an ionic strength of 0.1 has been studied by sedimentation equilibrium measurements in the temperature range from 5.0 to 25.0 °C. The thermodynamical treatment is also presented. The self-association of phycocyanin molecules proceeds with a decrease in the temperature. The concentration dependence of the apparent weight-average molecular weight of phycocyanin is interpreted in terms of a monomer\rightleftharpoonshexamer equilibrium system. It is clear from the thermodynamic parameters, Δ<I>G</I><SUP>0</SUP>, Δ<I>H</I><SUP>0</SUP>, and Δ<I>S</I><SUP>0</SUP>, that the self-association reaction is spontaneous in the solution of pH 5.4. In the association process, the formation of hydrophobic bonds is suggested by the large positive entropy change accompanying association reaction of phycocyanin molecules.
- 公益社団法人 日本化学会の論文
著者
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Mizuno Haruo
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Iso Naomichi
Department Of Food Science And Technology Tokyo University Of Fisheries
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Saito Takahide
Department of Chemistry Faculty of Science Tokyo University of Education
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Kitamura Ichiro
Department of Food Science and Technology, Tokyo University of Fisheries
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