Solution properties of phycoerythrin. I. Characterization of phycoerythrin.
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概要
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Phycoerythrin, one of the chromoproteins, from the red alga <I>Porphyra yezoensis</I> has been characterized by physicochemical measurement. The value of the sedimentation constant was 12s and it did not change with the change in pH from 5.4 to 6.8. This was assumed from the fact that the phycoerythrin did not undergo reversible dissociation-association reaction under these conditions. The weight average molecular weight of phycoerythrin was determined to be 2.2×10<SUP>5</SUP>, and the intrinsic viscosity of phycoerythrin was determined to be 2.29×10<SUP>−2</SUP> (10<SUP>−1</SUP> dm<SUP>3</SUP>/g). From these values, the molecular shape of phycoerythrin was inferred to be globular based on the Scheraga-Mandelkern equation. The SDS-polyacrylamide gel electrophoresis of phycoerythrin showed that the phycoerythrin consisted of two subunits with molecular weights of 3.8×10<SUP>4</SUP> and 2.3×10<SUP>4</SUP>. The sedimentation behavior of phycoerythrin suggested that the phycoerythrin solution was multicomponent. The phycoerythrin dissociated into two molecular species on treatment with sodium dodecyl sulfate. Their sedimentation constants were 12s and 2s. The 2s species was supposed to be phycoerythrin monomer.
- 公益社団法人 日本化学会の論文
著者
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Mizuno Haruo
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Ogawa Hiroo
Department Of Food Science And Technology Tokyo University Of Fisheries
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Iso Naomichi
Department Of Food Science And Technology Tokyo University Of Fisheries
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Saito Takahide
Department of Chemistry Faculty of Science Tokyo University of Education
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Ohzeki Fumiaki
Department of Food Science and Technology, Tokyo University of Fisheries
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Wang Zao
Department of Food Science and Technology, Tokyo University of Fisheries
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