Protection of isolated chromosome using bioactive beads toward physical and chemical stresses
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概要
- 論文の詳細を見る
Bioactive bead method has been developed as a novel gene transfer method for plant protoplasts. In this method, bioactive beads of several microns in size that entrap plasmid DNA are added to a protoplast suspension, after which PEG solution is added to the suspension. Using bioactive beads, transgene expression efficiency was improved. Moreover, yeast artificial chromosomes (YACs) have been introduced into yeast and tobacco cells and transformants were successfully obtained. In this study, we developed the method for entrapping metaphase chromosomes in bioactive beads by water-in-oil emulsification method. Chromosome entrapped bioactive beads were spherical and up to 90-100% encapsulation efficiency could be achieved when the concentration of the chromosomes to be mixed with sodium alginate solution was adjusted to 1×10^8/ml. Bioactive beads have protective effects on the entrapped chromosomes against salt treatment and physical shearing forces caused by vortex, pipetting, and experimental procedures involved in flow cytometry.
- 財団法人染色体学会の論文
著者
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FUKUI Kiichi
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Fukui Kiichi
Department Of Biotechnology Graduate School Of Engineering Osaka University
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DOI TOMOYUKI
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Murakawa Tomoko
Sasebo National College of Technology
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Doi Tomoyuki
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Fukui K
Grad. Sch. Of Eng. Osaka Univ.
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Hayashihara Kayoko
Department Of Biotechnology Graduate School Of Engineering Osaka University
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ITO Kohichi
Department of Periodontology, School of Dentistry, Nihon University
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Fukui Kiichi
Department Of Biotechnology Faculty Of Engineering Graduate School Of Osaka University
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Kajiyama Shin'ichiro
Department Of Biotechnology Graduate School Of Engineering Osaka University:school Of Biology-orient
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Kajiyama Shin'ichiro
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Ito Kohichi
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Kajiyama Shin-ichiro
Faculty Of Agriculture Okayama University
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Fukui Kiichi
Department Of Biotechnology Osaka University Graduate School Of Engineering
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