A Novel Transfection Method for Mammalian Cells Using Calcium Alginate Microbeads (TISSUE ENGINEERING)
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概要
- 論文の詳細を見る
The direct transfer of genetic materials into mammalian cells is an indispensable technique. We have developed calcium alginate (CA) microbeads which can deliver plasmid DNAs and yeast artificial chromosomes into plant and yeast cells. In this paper, we demonstrate the effective transfection of mammalian cells by CA microbeads immobilizing plasmid DNAs. The transfection was performed using the pEGFP-C1 plasmid containing the cytomegalovirus (CMV) promoter and enhanced green fluorescent protein (EGFP) gene. The transient expression of EGFP was observed 24 h after transfection. The expression efficiency was maximum when the concentration of sodium alginate was 1% and the amount of plasmid DNA was increased to 100 μg. The expression efficiency of our method using CA microbeads is 2-10 times higher than that of the polyethylene glycol (PEG) method. Our results suggest that the CA microbead mediated transfection of mammalian cells effectively delivers genetic materials into mammalian suspension cells.
- 社団法人日本生物工学会の論文
- 2004-03-25
著者
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Nagamori Eiji
Department Of Biotechnology School Of Engineering Nagoya University
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Nagamori Eiji
Toyota Central R & D Labs. Inc.
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Nagamori E
Osaka Univ. Osaka Jpn
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Nagamori Eiji
Biotechnology Laboratory Toyota Central R&d Labs Inc.
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FUKUI Kiichi
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Nagamori Eiji
Toyota Central R&d Labs. Inc. 41-1 Yokomichi Nagakute Aichi 480-1192 Jpn
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Matsunaga Sachihiro
Department Of Integrated Biosciences Graduate School Of Frontier Sciences University Of Tokyo
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HIGASHI TSUNEHITO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Fukui K
Grad. Sch. Of Eng. Osaka Univ.
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Higashi Tsunehito
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Matsunaga Sachihiro
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Matsunaga Sachihiro
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Sone Takefumi
Imbd Osaka Univ.
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SONE TAKEFUMI
Research Institute for Microbial Diseases, Osaka University
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Fukui Kiichi
Department Of Biotechnology Faculty Of Engineering Graduate School Of Osaka University
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Matsunaga Sachihiro
Department Of Applied Biological Science Faculty Of Science And Technology Tokyo University Of Science
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Nagamori Eiji
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Higashi Tsunehito
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science:(Present office)Department of Cellular Pharmacology, Graduate School of Medicine, Hokkaido University
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HIGASHI Tsunehito
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science
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