Semi-Automatic Laser Beam Microdissection of the Y Chromosome and Analysis of Y Chromosome DNA in a Dioecious Plant, Silene latifolia
スポンサーリンク
概要
- 論文の詳細を見る
Silene latifolia has heteromorphic sex chromosomes, the X and Y chromosomes. The Y chromosome, which is thought to carry the male determining gene, was isolated by UV laser microdissection and amplified by degenerate oligonucleotide-primed PCR. In situ chromosome suppression of the amplified Y chromosome DNA in the presence of female genomic DNA as a competitor showed that the microdissected Y chromosome DNA did not specifically hybridize to the Y chromosome, but hybridized to all chromosomes. This result suggests that the Y chromosome does not contain Y chromosome-enriched repetitive sequences. A repetitive sequence in the microdissected Y chromosome, RMY1, was isolated while screening repetitive sequences in the amplified Y chromosome. Part of the nucleotide sequence shared a similarity to that of X-43.1, which was isolated from microdissected X chromosomes. Since fluorescence in situ hybridization analysis with RMY1 demonstrated that RMY1 was localized at the ends of the chromosome, RMY1 may be a subtelomeric repetitive sequence. Regarding the sex chromosomes, RMY1 was detected at both ends of the X chromosome and at one end near the pseudoautosomal region of the Y chromosome. The different localization of RMY1 on the sex chromosomes provides a clue to the problem of how the sex chromosomes arose from autosomes.
- 日本植物生理学会の論文
著者
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MATSUNAGA Sachihiro
Department of Biotechnology, Graduate School of Engineering, Osaka University
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SAKAI Atsushi
Department of Materials Science and Engineerneering, Interdisciplinary Graduate School of Science an
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Sakai A
Univ. Tokyo Tokyo Jpn
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KAWANO Shigeyuki
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo
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KUROIWA Tsuneyoshi
Department of Biological Sciences, Graduate School of Science, University of Tokyo
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NAKAO Shunsuke
Dept. Biol. Sci., Grad. Schl. Sci., Univ. Tokyo
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Michimoto Takeshi
Department of Biological Sciences, Graduate School of Science, University of Tokyo
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Higashiyama Tetsuya
Department of Biological Sciences, Graduate School of Science, University of Tokyo
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Nakao Shunsuke
Department of Biological Sciences, Graduate School of Science, University of Tokyo
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Matsunaga S
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Matsunaga Sachihiro
Department Of Integrated Biosciences Graduate School Of Frontier Sciences University Of Tokyo
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Kuroiwa T
Rikkyo Univ. Tokyo Jpn
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Kawano S
Department Of Integrated Biosciences Graduate School Of Frontier Sciences University Of Tokyo
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Kawano Shigeyuki
Department Of Biology Faculty Of Science University Of Tokyo
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Kawano Shigeyuki
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Sakai A
Nara Women's Univ. Nara Jpn
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Kuroiwa Tsuneyoshi
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Matsunaga Sachihiro
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Matsunaga Sachihiro
Department Of Biological Sciences School Of Science University Of Tokyo
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Kuroiwa T
Research Information Center For Extremophiles Graduate School Of Science Rikkyo (st. Paul's) Un
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Sakai Atsushi
Department Of Biological Science Faculty Of Science Nara Women's University
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河野 重行
Department Of Integrated Biosciences Graduate School Of Frontier Sciences University Of Tokyo
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Kawano Shigeyuki
Department Of Integrated Sciences Graduate School Of Frontier Sciences University Of Tokyo
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Kawano Shigeyuki
Department Of Plant Science Graduate School Of Science University Of Tokyo
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Nakao Shunsuke
Department Of Biological Sciences Graduate School Of Sciences University Of Tokyo
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Takahashi H
Division Of Biological Science Graduate School Of Science Nagoya University
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Michimoto Takeshi
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Kuroiwa Tsuneyoshi
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
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Sakai Atsushi
Department Of Biochemistry Faculty Of Veterinary Medicine Hokkaido University
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Higashiyama Tetsuya
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Matsunaga Sachihiro
Department Of Applied Biological Science Faculty Of Science And Technology Tokyo University Of Science
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