Transcriptional Activity of Morphologically Intact Proplastid-Nuclei (Nucleoids) Isolated from Tobacco Cultured Cells
スポンサーリンク
概要
- 論文の詳細を見る
The transcriptional activity of morphologically intact proplastid-nuclei (synonymous with proplastid-nucleoids) isolated from cultured tobacco cells (line BY-2) was examined by an assay of transcription in vitro and Southern hybridization. The isolated proplastid-nuclei incorporated about 7 pmol of UTP into RNA per microgram of DNA during a 60-min incubation. This incorporation of UTP was identified as synthesis of RNA that was dependent on DNA-dependent RNA polymerase. Heparin, a potent inhibitor of initiation of transcription, had no inhibitory effect, suggesting that the transcriptional activity in the isolated proplastid-nuclei is primarily due to chain elongation of nascent transcripts. The compactly organized, chromatin-like structure of the proplastid-nuclei was maintained during the assay of transcription in vitro under suitable conditions. Southern hybridization studies revealed that the pattern of transcripts generated in vitro by the isolated proplastid-nuclei were similar to that of transcripts produced in a lysed proplastid system and that of RNA accumulated in the living cells. Therefore, the system for the assay of transcription in vitro using isolated proplastid-nuclei seems to provide a suitable method for analyzing the relationship between the transcriptional regulation of plastid genes and the structural changes in plastid-nuclei.
- 日本植物生理学会の論文
著者
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NEMOTO Yasuyuki
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology
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SAKAI Atsushi
Department of Materials Science and Engineerneering, Interdisciplinary Graduate School of Science an
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KAWANO Shigeyuki
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo
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KUROIWA Tsuneyoshi
Department of Biological Sciences, Graduate School of Science, University of Tokyo
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Kawano Shigeyuki
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Sakai A
Nara Women's Univ. Nara Jpn
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Kuroiwa Tsuneyoshi
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Yamashita Hirofumi
Department Of Biology Faculty Of Science University Of Tokyo
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Sakai Atsushi
Department Of Biological Science Faculty Of Science Nara Women's University
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Nemoto Y
Dept.biotech. Fac.tech. Tokyo Univ.agric.tech.
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Nemoto Yasuyuki
Department Of Biology Faculty Of Science University Of Tokyo
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Kuroiwa Tsuneyoshi
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
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Sakai Atsushi
Department Of Biochemistry Faculty Of Veterinary Medicine Hokkaido University
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