Intracellular Fate of 2-NBDG, a Fluorescent Probe for Glucose Uptake Activity, in Escherichia coli Cells
スポンサーリンク
概要
- 論文の詳細を見る
A fluorescent derivative of D-glucose, 2-NBDG, which was previously developed for the evaluation of glucose uptake activity by living cells, was used on Escherichia coli cells and its fate after incorporation in the cells was investigated. 2-NBDG was converted to another fluorescent derivative (2-NBDG metabolite) immediately after it was taken by E. coli cells. This 2-NBDG metabolite was then decomposed to non-fluorescent forms. 2-NBDG metabolite was decomposed into the original 2-NBDG by G6Pase with concurrent liberation of inorganic phosphate. Furthermore, FAB/MS analysis showed that its molecular weight was 420, the same value as that of 2-NBDG 6-phosphate. These indicate 2-NBDG metabolite should be 2-NBDG 6-phosphate. Based on these results, the feasibility of 2-NBDG as a fluorescent non-toxic probe for glucose uptake activity and its application to viability assessment of various living systems are discussed.
- 社団法人日本農芸化学会の論文
- 1996-11-23
著者
-
Yoshioka Kazuaki
Department Of Physiology Kanazawa University
-
Yoshioka Kazuaki
Department Of Molecular Cell Signaling Tokyo Metropolitan Institute For Neuroscience
-
ABE Hiroshi
Department of Pathology, Juntendo University School of Medicine
-
Abe Hiroshi
Department Of Cardiology National Cardiovascular Center
-
Oh K‐b
Natural Products Research Institute Seoul National University
-
SAITO MIKAKO
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology
-
MATSUOKA HIDEAKI
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology
-
OH KI-BONG
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology
-
NEMOTO Yasuyuki
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology
-
NATSUME Masahiro
Department of Applied and Biological Science, Faculty of Agriculture, Tokyo University of Agricultur
-
松岡 英明
東京農工大学大学院工学府生命工学専攻
-
Yoshioka K
Inst. Physical And Chemical Res.(riken) Saitama Jpn
-
Saito Mikako
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology
-
Saito M
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology
-
Matsuoka Hideaki
Department Of Biotechnology And Life Science Faculty Of Technology Tokyo University Of Agriculture A
-
SASAKI Mami
RIKEN Plant Science Center
-
Abe Hiroshi
Department Of Anatomy Akita University School Of Medicine
-
Nemoto Y
Dept.biotech. Fac.tech. Tokyo Univ.agric.tech.
-
Nemoto Yasuyuki
Department Of Biology Faculty Of Science University Of Tokyo
-
Natsume Masahiro
Department Of Agricultural Chemistry Nagoya University
-
Natsume Masahiro
Department Of Applied And Biological Science Faculty Of Agriculture Tokyo University Of Agriculture
-
Abe Hiroshi
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
-
Abe Hiroshi
Department Of Applied And Biological Science Faculty Of Agriculture Tokyo University Of Agriculture
-
Yoshioka Kazuaki
Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology
-
Oh Ki-Bong
Department of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University
関連論文
- Inhibitory Effects of Octreotide Acetate, a Somatostatin Analog, on Spontaneous Chronic Pancreatitis in WBN/Kob Rats
- PJ-504 Aortic Pulse Wave Velocity can Predict Left Ventricular Diastolic Dysfunction in a Community-based Population(PJ085,Heart Failure (Pathophysiology) (M),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Society
- 5 A Role of Endoplasmic Reticulum Stress in the Pathophysiology of Atherosclerotic Plaques Associated with Acute Coronary Syndrome(Vulnerable Patient/Vulnerable Plaque in Acute Coronary Syndrome,Plenary Session 2 (PL-2) (H),The 73rd Annual Scientific Meet
- Inhibition of Cardiac Remodeling by Pravastatin Is Associated with Amelioration of Endoplasmic Reticulum Stress
- Plasma Adiponectin Is Associated with Plasma Brain Natriuretic Peptide and Cardiac Function in Healthy Subjects
- PJ-763 Improvement of Left Ventricular Ejection Fraction is a Predictor for Cardiac Events in Patients with Acute Myocardial Infarction(Acute myocardial infarction, clinical(diagnosis / treatment)(12)(IHD),Poster Session(Japanese),The 72nd Annual Scientif
- PJ-308 Miglitol, a Novel Alpha-Glucosidase Inhibitor, Improves Left Ventricular Function in Dogs With Pacing-Induced Heart Failure(Heart failure, basic(06)(M),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-110 Transmural Heterogeneity of Global Gene Expression Profiles in Canine Failing Ventricular Myocardium(Heart failure, basic(05)(M),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- OJ-091 Activation of AMP-activated Protein Kinase by Metformin Prevents Progression of Heart Failure in Dogs(Heart failure, basic(02)(M),Oral Presentation(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-504 Aortic Pulse Wave Velocity can Predict Left Ventricular Diastolic Dysfunction in a Community-based Population(PJ085,Heart Failure (Pathophysiology) (M),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Society
- PE-482 Does Assessment of Atrial Contractile Properties Predict Paroxysmal Atrial Fibrillation? : A Color Doppler Strain Rate Imaging Study(PE081,Arrhythmia, Others (Clinical/Diagnosis/Treatment) 4 (A),Poster Session (English),The 73rd Annual Scientific M
- OE-128 Left Ventricular Global Strain and Dyssynchrony Demonstrated by Speckle Tracking Echocardiography : A Comparison of Apical and Septal Right Ventricular Pacing(OE22,Echo/Doppler (Myocardium) (I),Oral Presentation (English),The 73rd Annual Scientific
- 5 A Role of Endoplasmic Reticulum Stress in the Pathophysiology of Atherosclerotic Plaques Associated with Acute Coronary Syndrome(Vulnerable Patient/Vulnerable Plaque in Acute Coronary Syndrome,Plenary Session 2 (PL-2) (H),The 73rd Annual Scientific Meet
- PJ-763 Improvement of Left Ventricular Ejection Fraction is a Predictor for Cardiac Events in Patients with Acute Myocardial Infarction(Acute myocardial infarction, clinical(diagnosis / treatment)(12)(IHD),Poster Session(Japanese),The 72nd Annual Scientif
- PJ-218 Bachmann's Bundle Pacing Gets an Advantage over Right Atrial Appendage Pacing on Atrial Contraction Synchrony : A Strain Rate Imaging Study(Arrhythmia, therapy(24)(A),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circu
- OJ-247 Influence of Preload Changes Mediated by Upper Body Tilting Position on Left Ventricular Diastolic Indices in chronic heart failure subjects(Echo / Doppler(04)(I),Oral Presentation (Japanese),The 72nd Annual Scientific Meeting of the Japanese Circu
- 3 Management of Hyperphosphatemia is an Important Novel Therapeutic Target in Chronic Heart Failure(Symposium 11 (SY-11) (M) Stroke and Cardiorenal Complications in Hypertension,Special Program,The 72nd Annual Scientific Meeting of the Japanese Circulatio
- 3 Potential Pharmacological Adjunctive Therapy for Acute Myocardial Infarction : Lessons from J-WIND trials(Plenary Session 2 (PL-2) (IHD) Myocardial Protection Strategy after Reperfusion Therapy in Acute Coronary Syndrome,Special Program,The 72nd Annual
- Atorvastatin Slows the Progression of Cardiac Remodeling in Mice with Pressure Overload and Inhibits Epidermal Growth Factor Receptor Activation
- Long-Term Oral Administration of Dipyridamole Improves Both Cardiac and Physical Status in Patients with Mild to Moderate Chronic Heart Failure : A Prospective Open-Randomized Study
- Impact of Adenosine Receptor Signaling and Metabolism on Pathophysiology in Patients with Chronic Heart Failure
- PE-342 Novel Point-of-Care Test for Oxidative Stress, ROM Test, Reflects Elevated Oxidation Levels in the Patients with Chronic Heart Failure(Heart failure, clinical-09, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OJ-250 A Novel Role of Histamine H2 Receptors in the Pathophysiology of Canine Heart Failure Induced by Sustained Rapid Pacing(Heart failure, basic-2, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- FRS-092 Chronic Stimulation of Adenosine A2b Receptors Begun after Myocardial Infarction Prevents Cardiac Remodeling in Rats(Heart Failure, Cardiac Remodeling and Cardiomyopathy 2, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-334 Measurement of the Plasma Adiponectin Level is Useful for the Prediction of Metabolic Syndrome : Result from KING Study(PJ056,Preventive Medicine/Epidemiology/Education 2 (H),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japan
- PJ-197 Common Variants in and around the Adiponectin Gene Associated with Plasma Adiponectin Level : Result from KING Study(PJ033,Preventive Medicine/Epidemiology/Education 1 (H),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese
- PJ-192 Genetic Determinants of Plasma Resistin Levels in a Japanese Aged Cohort (KING Study)(PJ033,Preventive Medicine/Epidemiology/Education 1 (H),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- Comparison of the Effects of Telmisartan and Olmesartan on Home Blood Pressure, Glucose, and Lipid Profiles in Patients with Hypertension, Chronic Heart Failure, and Metabolic Syndrome
- 4 Current Status and Surrounding Issues of Clinical Trials in Japan : Lesson and Proposal from our Experience of the Clinical Trials(Clinical Trials Update in Japan and Future Perspective, The 71st Annual Scientific Meeting of the Japanese Circulation Soc
- Abnormal Glucose Tolerance Contributes to the Progression of Chronic Heart Failure in Patients with Dilated Cardiomyopathy
- Combined Surgical and Endovascular Treatment of Infected Pseudoaneurysm After Carotid Endarterectomy : Case Report
- Adult Chiari Type I Malformation Presenting as Dysphagia. - A Case Report -
- Frontal Lobe Infarction Due to Hemodynamic Change after Surgical Revascularization in Moyamoya Disease : Two Case Reports
- Anterior Approach for Dumbbell Type Cervical Neurinoma
- Telemedicine in Neurosurgery Using Internetional Digital Telephone Services Between Japan and Malaysia : Technical Note
- Intracellular Fate of 2-NBDG, a Fluorescent Probe for Glucose Uptake Activity, in Escherichia coli Cells
- PJ-506 Comparing Effects of Loop Diuretics with Antialdosteronergic Properties Versus Pure Loop Diuretics in Mildly Symptomatic Patients with Chronic Heart Failure(Heart failure, clinical(17)(M),Poster Session(Japanese),The 72nd Annual Scientific Meeting
- PE-166 Superoxide-Dependent Activation of Cathepsin System is Associated with Hypertensive Myocardial Remodeling and Represents a Target for AT1R Blocker Therapy(Cardiovascular pharmacology, basic/clinical(03)(H),Poster Session(English),The 72nd Annual Sc
- OE-366 Increased ^Tc-Sestamibi Washout Reflects Impaired Myocardial Contractile Reserve and Prolonged Relaxation in Patients with Nonobstructive Hypertrophic Cardiomyopathy(Nuclear cardiology(01)(I),Oral Presentation(English),The 72nd Annual Scientif
- PJ-494 Contraction Response to Dobutamine Could Predict Altered Myocardial Contractile Regulatory Proteins Expressions in Mildly Symptomatic Patients with Dilated Cardiomyopathy(Heart failure, clinical-14, The 71st Annual Scientific Meeting of the Japanes
- 1 Evidence for Human Atrial Natriuretic Peptide and Nicorandil as an Adjunct to Reperfusion Therapy for Acute Myocardial Infarction : J-WIND Trials(Late Breaking Clinical Trials in Japan, The 71st Annual Scientific Meeting of the Japanese Circulation Soci
- 3 Human Atrial Natriuretic Peptide and Nicorandil as an Adjunct to Reperfusion Therapy for Acute Myocardial Infarction : Lessons from J-WIND Trials(Discovery of Adjunctive Therapies for Acute Myocardial Infarction-From Bench to Bedside-, The 71st Annual S
- 4 Abnormal Glucose Tolerance as the Risk of Progression of Heart Failure or the Onset of Coronary Heart Disease(Diabetic Cardiovascular Complications and Therapeutic Strategy, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- 6 Experience of Large-Scale Trial Using ANP or Nicorandil as an Adjunct to Percutaneous Coronary Intervention for Acute Myocardial Infarction(New Horizon in Coronary Interventions for Acute Coronary Syndrome, The 71st Annual Scientific Meeting of the Japa
- The ATP-sensitive Potassium Channel Opener, Nicorandil, Reduces No-reflow Phenomenon and Infarct Size in the Canine Heart
- Adenosine-Induced Cardiac Gene Expression of Ischemic Murine Hearts Revealed by cDNA Array Hybridization
- -331-QUANTITATIVE ASSESSMENT OF CARDIAC REJECTION IN HETEROTOPIC HEART TRANSPLANTATION USING ^111-ANTIMYOSIN FAB
- AUGMENTED ENERGY CONSUMPTION DURING EARLY SYSTOLE AS A MECHANISM OF CYCLICAL CHANGES IN HIGH-ENERGY PHOSPHATES IN MYOCARDIUM ASSESSED BY PHOSPHORUS NUCLEAR MAGNETIC RESONANCE
- LEFT VENTRICULAR SYSTOLIC FUNCTION OF in situ DENERVATED HEART AND OF COMPETITIVE BEATS WITH A PULSATILE PUMP : Cardiac Function (I) : FREE COMMUNICATIONS (I) : PROCEEDINGS OF THE 49th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- DEVELOPMENT OF QUANTITATIVE ANALYSIS OF PLASMA THROMBOXANE B_2 BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY
- THE FUNCTIONAL CHARACTERISTICS OF CULTURED RAT MESANGIAL CELL
- THE ROLE OF CORONARY ARTERY SPASM AND CORONARY ARTERY SCLEROSIS IN UNSTABLE ANGINA : Ischemic Heart Disease (V) : V : 48 Annual Scientific Meeting, Japanese Circulation Society
- NEW TYPES OF ARRHYTHMIA REVERSION SYSTEMS AGAINST TACHYARRHYTHMIA : Arrhythmias (III) : V : 48 Annual Scientific Meeting, Japanese Circulation Society
- PURIFICATION AND CHARACTERIZATION OF PHOSPHOLAMBAN FROM CARDIAC SARCOPLASMIC RETICULUM : Cardiac Metabolism (II) : V : 48 Annual Scientific Meeting, Japanese Circulation Society
- EFFECT OF A TXA_2 SYNTHETASE INHIBITOR ON CYCLICAL REDUCTIONS OF CORONARY FLOW OF CANINE CORONARY CIRCULATION : POSSIBLE ROLE OF PGH_2 "STEAL" PHENOMENON BETWEEN PLATELET AND VASCULAR TISSUE : Ischemic Heart Disease (II), Coronary Circulation : II : 48 An
- THROMBOXANE A_2 AS AN ENHANCING FACTOR IN CORONARY ARTERIAL SPASM IN VARIANT ANGINA : Ischemic Heart Disease (II), Coronary Circulation : II : 48 Annual Scientific Meeting, Japanese Circulation Society
- ARTERIOGRAPHIC FEATURES OF ANGINA PECTORIS ASSOCIATED WITH ST SEGMENT DEPRESSION DURING CORONATY ARTERIAL SPASM
- DETERMINATION OF PLASMA THROMBOXANE B_2 BY RADIOIMMUNOASSAY : PROCEEDINGS OF THE 47th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY : Myocardial Metabolism Biochemistry
- EFFECT OF PHOSPHOLAMBAN PHOSPHORYLATION ON Ca^-DEPENDENT ATPase OF SOLUBILIZED CARDIAC SARCOPLASMIC RETICULUM : PROCEEDINGS OF THE 47th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY : Myocardial Metabolism Biochemistry
- PURIFICATION OF PHOSPHOLAMBAN FROM CARDIAC SARCOPLASMIC RETICULUM : PROCEEDINGS OF THE 47th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY : Myocardial Metabolism Biochemistry
- Primary Yolk Sac Tumor Within the Lateral Ventricle : Case Report
- Complication Caused by Use of Fibrin Glue in Vessel Transposition for Trigeminal Neuralgia
- CLINICAL SIGNIFICANCE OF EXCESSIVELY GENERATED THROMBOXANE A_2 DURING THE ACUTE PHASE OF MYOCARDIAL INFARCTION : Ischemic Heart Disease (II), Coronary Circulation : II : 48 Annual Scientific Meeting, Japanese Circulation Society
- CLINICAL USEFULNESS OF FOURIER PHASE ANALYSIS OF RIGHT VENTRICULAR WALL MOTION IN THE DETECTION OF PROXIMAL RIGHT CORONARY ARTERY STENOSIS : Doppler, RI : I : 48 Annual Scientific Meeting, Japanese Circulation Society
- ASSESSMENT OF INFARCT SITE AND SIZE IN ACUTE MYOCARDIAL INFARCTION FROM PRECORDIAL ST POTENTIALS : PROCEEDINGS OF THE 47th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY : Vectorcardiography Mapping
- SYSTEMIC AND CORONARY HEMODYNAMICS DURING ANGINA : A COMPARISON DURING ANGINA INDUCED BY EXERCISE AND BY ATRIAL PACING : PROCEEDINGS OF THE 47th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY : Exercise Test
- CARDIAC FUNCTION DURING DYNAMIC EXERCISE IN PATIENTS WITH MYOCARDIAL INFARCTION ASSESSED BY MULTIPLE GATED CARDIAC POOL IMAGING : PROCEEDINGS OF THE 47th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY : Exercise Test
- PJ-092 Activation of Adenosine A1 Receptors Inhibits Aldosterone Synthesis in Adrenal Glands(Metabolism/Biochemistry/Energetics-3 (M) PJ16,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- STUDIES ON HUCKEL'S MOLECULAR-ORBITAL CALCULATION (3dπ-2pπ) OF THE SULFAMYL PART OF THIAZIDE DIURETICS
- PE-342 Dobutamine-Induced Mechanical Alternans Could be a Useful Predictor of Poor Prognosis in Ambulatory Patients with Idiopathic Dilated Cardiomyopathy(Heart failure, clinical(10)(M),Poster Session(English),The 72nd Annual Scientific Meeting of the Jap
- Aggravation of Brainstem Symptoms Caused by a Large Superior Cerebellar Artery Aneurysm after Embolization by Guglielmi Detachable Coils
- Multiple Dural Arteriovenous Fistulas
- PJ-672 G_ and G_q Coordinately Mediate S1P_2-induced Inhibition of Rac and Migration in Vascular Smooth Muscle in Rho-dependent Manner(PJ113,Atherosclerosis, Basic 2 (IHD),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese
- OE-181 Class-IIα Phosphoinositide 3-Kinase is an Essential Regulator of Ca^-Dependent Rho Activation, Myosin Phosphatase and Contraction in Vascular Smooth Muscle Cells(Vascular smooth muscle-1, The 71st Annual Scientific Meeting of the Japanese Circu
- Dural band pathology in syringomyelia with Chiari type I malformation
- Bone Window Computed Tomography for Detection of Dural Defect Associated with Cervical Ossified Posterior Longitudinal Ligament
- Immunohistochemical analysis of giant cell glioblastoma
- PJ-263 Tc-99m-sestamibi Washout Rate Could be Reflected Delayed Myocardial Relaxation in Patients with Hypertrophic Cardiamyopathy(Nuclear cardiology-7 (I) PJ45,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circu
- Dystrophic Axonal Formation (Spheroid Body) in Central Neurocytoma : Case Report
- Desmoplastic Infantile Ganglioglioma : Case Report
- Clinicopathological Study of Multiple Gliomas : Report of Three Cases
- OE-299 Myocardium Strain during Dobutamine Stress Echocardiography Discriminate Ischemic Heart Disease from Dilated Cardiomyopathy(Echo/Doppler-03, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- Effect of Introducing a Lactone Group into Typhasterol and Teasterone to Promote Rice Lamina Inclination
- Magnetic Resonance Angiography for Detection of Occlusive Neck and Proximal Vascular Diseases
- Aneurysm of the Anterior Communicating Artery Associated with Accessory Middle Cerebral Artery : Case Report
- Clinical Application of Angioscopy During Carotid Endarterectomy for Patients with Internal Carotid Artery Stenosis
- Magnetic Resonance Findings in Spontaneous Dissection of the Cervical Internal Carotid Artery : Case Report
- Cavernous Angioma of the Cerebellum and Cerebellar Atrophy : Case Report
- Clinical Application of Proton Magnetic Resonance Spectroscopy to Cerebral Ischemia
- Structure-activity Relationship of Pamamycins : Effect of Side Chain Length on Aerial Mycelium-inducing Activity
- Isolation and structural properties of aerial mycelium differentiation-inhibitory substances against Streptomyces scabiei causing potato common scab
- P-72 BIOSYNTHETIC PATHWAY OF PAMAMYCIN, A NITROGEN-CONTAINING POLYKETIDE
- Nitrogen Incorporation in the Biosynthetic Pathway of the Nitrogen-containing Polyketide, Pamamycin in Streptomyces alboniger
- Biosynthetic Origin of the Carbon Skeleton and Nitrogen Atom of Pamamycin-607, a Nitrogen-Containing Polyketide
- Relationship between Response to and Production of the Aerial Mycelium-inducing Substances Pamamycin-607 and A-factor(Microbiology & Fermentation Technology)
- De-N-methylpamamycin-593A and B, New Pamamycin Derivatives Isolated from Streptomyces alboniger
- Low Molecular-weight G-actin Binding Proteins Involved in the Regulation of Actin Assembly during Myofibrillogenesis
- Immunolocalization of the β Subunit of Prolyl Hydroxylase in the Pancreas
- ATP-and Adenosine-Mediated Signaling in the Central Nervous System : Purinergic Receptor Complex : Generating Adenine Nucleotide-Sensitive Adenosine Receptors
- Deletion of sphingosine-1-phosphate receptor-2 inhibits proinflammatory activities of macrophages and atherosclerosis in apoE-deficient mice