DEVELOPMENT OF QUANTITATIVE ANALYSIS OF PLASMA THROMBOXANE B_2 BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY
スポンサーリンク
概要
- 論文の詳細を見る
In order to diagnose patients in thrombotic state, it is quite important to detect increased concentration of plasma thromboxane B_2 (TXB_2), a stable catabolite of TXA_2. To determine plasma TXB_2 levels with high sensitivity and selectivity, we employed gas chromatography-mass spectrometry (GC/MS). The trimethylsilyl (TMS) ether derivatives conventionally employed in GC/MS analysis of prostanoids are not suitable for quantitation of plasma prostanoids, because the mass spectra are deficient in ions with high intensity in the high mass range and TMS ether derivatives are sensitive to moisture. To solve these problems we employed tert-butyldimethylsilyl (t-BDMS) ether derivatives, based on the observation that t-BDMS ether derivatives afforded abundant ions at [M-57]^+ and showed good hydrolytic stability. The reaction conditions of tert-butyldimethylsilylation were also examined to optimize the selected ion monitoring response. The t-BDMS ether derivative of prostanoids were successfully analyzed with a short capillary column with a relatively large diameter, with maintaining good separation. In conjunction with the use of reversed-phase high performance liquid chromatography as purification procedure, a sensitive and reproducible stable isotope dilution assay of plasma TXB_2 was developed. The values obtained by this method correlated well with those obtained by the radioimmunoassay we have developed.
- 社団法人日本循環器学会の論文
- 1985-03-20
著者
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Minamino Takazo
Department Of Internal Medicine And Therapeutics Osaka University Graduate School Of Medicine
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Abe Hiroshi
Department Of Cardiology National Cardiovascular Center
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Manabe Hisao
Department of Cardiovascular Surgery National Cardiovascular Center
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Abe Hiroshi
Cardiovascular Division Osaka National Hospital
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TADA MICHIHIKO
The Division of Cardiology, First Department of Medicine
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MATSUDA HISAO
The Division of Cardiology, First Department of Medicine
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KUZUYA TSUNEHIKO
The Division of Cardiology, First Department of Medicine
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INUI MAKOTO
The Division of Cardiology, First Department of Medicine
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ABE HIROSHI
The Division of Cardiology, First Department of Medicine
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Inui Makoto
First Department Of Medicine And Department Of Pathophysiology Osaka University School Of Medicine
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Inoue Michitoshi
The First Department Of Medicine Osaka University Medical School
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Inoue Michitoshi
First Department Of Medicine And Department Of Pathophysiology Osaka University School Of Medicine
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Tada Michihiko
The Division Of Cardiology Sakurabashi Watanabe Hospital
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