D-Lactic Acid Production by Metabolically Engineered Saccharomyces cerevisiae(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
Poly D-lactic acid is an important polymer because it improves the thermostability of poly L-lactic acid by the stereo complex formation. We constructed a metabolically engineered Saccharomyces cerevisiae that produces D-lactic acid efficiently. In this recombinant, the coding region of pyruvate decarboxylase 1 (PDC1) was completely deleted, and two copies of the D-lactate dehydrogenase (D-LDH) gene from Leuconostoc mesenteroides subsp. mesenteroides strain NBRC3426 were introduced into the genome. The D-lactate production reached 61.5 g/l, the amount of glucose being transformed into D-lactic acid being 61.2% under neutralizing conditions. Additionally, the yield of free D-lactic acid was also shown to be 53.0% under non-neutralizing conditions. It was confirmed that D-lactic acid of extremely high optical purity of 99.9% or higher. Our finding obtained the possibility of a new approach for pure D-lactic acid production without a neutralizing process compared with other techniques involving lactic acid bacteria and transgenic Escherichia coli.
- 社団法人日本生物工学会の論文
- 2006-02-25
著者
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Kitamoto Katsuhiko
Department of Biotechnology, The University of Tokyo
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Nagamori Eiji
Department Of Biotechnology School Of Engineering Nagoya University
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Nagamori Eiji
Toyota Central R & D Labs. Inc.
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Nagamori E
Osaka Univ. Osaka Jpn
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Nagamori Eiji
Biotechnology Laboratory Toyota Central R&d Labs Inc.
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Kitamoto Katsuhiko
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Takahashi Haruo
Biotechnology Laboratory Toyota Central R&d Labs Inc.
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ISHIDA Nobuhiro
Biotechnology Laboratory, Toyota Central R&D Labs Inc.
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SAITOH Satoshi
Toyota Biotechnology & Afforestation Laboratory, Toyota Motor Co.
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ONISHI Toru
Toyota Biotechnology & Afforestation Laboratory, Toyota Motor Co.
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TOKUHIRO Kenro
Biotechnology Laboratory, Toyota Central R&D Labs Inc.
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Suzuki Tomiko
Biotechnology Laboratory, Toyota Central R&D Labs Inc.
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Saitoh Satoshi
Toyota Biotechnology & Afforestation Laboratory Toyota Motor Co.
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Onishi Toru
Toyota Biotechnology & Afforestation Laboratory Toyota Motor Co.
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Suzuki Tomiko
Biotechnology Laboratory Toyota Central R&d Labs Inc.
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Ishida Nobuhiro
Biotechnology Laboratory Toyota Central R&d Labs Inc.
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Tokuhiro Kenro
Biotechnology Laboratory Toyota Central R&d Labs Inc.
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Kitamoto Katsuhiko
Department Of Applied Biological Chemistry And Department Of Applied Biological Engineering Graduate
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Ishida Nobuhiro
Biotechnology Laboratoiy Toyota Central R&d Labs Inc.
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Takahashi Haruo
Biotechnology Laboratoiy, Toyota Central R&D Labs Inc.
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