Ionic liquid/water interfacial localization of a green fluorescent protein fused to a tryptophan-rich peptide(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
- 論文の詳細を見る
We report that several tryptophan-rich peptides exhibit an affinity for a hydrophobic ionic liquid (IL) (1-ethyl-3- methylimidazolium bis-trifluoromethanesulfonyl imide), and that green fluorescent protein (GFP) fused to a peptides, "SSSWWSWWWW" (SW1) or "SWWWWSWWWW" (SW2), containing serine (S) and tryptophan (W) at the C terminus localized at the IL/water interface. While GFPs without W-rich peptide distributed only in water phase, SW1- and SW2-GFPs were accumulated at the interface. The localization of SW1-GFP showed biphasic behavior, and most distinctive localization was observed at 7.1μM. The localization of SW2-GFP presumably occurred at largely lower concentration (&le:0.5μM) than that of SW1-GFP, which difference was due to the higher hydrophobicity of SW2 peptide.
著者
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Takahashi Haruo
Biotechnology Laboratory Toyota Central R&d Labs Inc.
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Kumagai Izumi
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Kumagai Izumi
Department Of Biochemistry And Engineering Graduate School Of Engineering Tohoku University
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Takahashi Haruo
Toyota Central R & D Lab. Inc.
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Umetsu Mitsuo
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Yamanishi Mamoru
Matsuyama Research Group Toyota Central R&d Labs. Inc.
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Takahashi Haruo
Biotechnology Laboratory Toyota Central R&d Labs. Inc.
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Matsuyama Takashi
Matsuyama Research Group Toyota Central R&d Labs. Inc.
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Yamanishi Mamoru
Toyota Central R&d Lab.
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Ikawa Taiji
Toyota Central R&d Lab.
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Matsuyama Takashi
Toyota Central R&d Lab.
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Domyoung Kim
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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Ishida Nobuhiro
Toyota Central R&D Lab.
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Domyoung Kim
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Umetsu Mitsuo
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University:center For Interdisciplinary Research Tohoku University
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Ishida Nobuhiro
Toyota Central R&d Lab.
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Ishida Nobuhiro
Toyota Central R&D lab
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