Efficient Construction of a Diabody Using a Refolding System: Anti-Carcinoembryonic Antigen Recombinant Antibody Fragment.

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概要

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• Recombinant fragments of the variable region of antibodies are useful in many experimental and clinical applications. However, it can be difficult to obtain these materials in soluble form after their expression in bacteria. Here, we report an efficient procedure for preparing several variable-domain fragments (Fv), single-chain Fv (scFv), and a diabody (the smallest functional bispecific antibody) of anti-carcinoembryonic antigen (CEA) antibody by overexpression in Escherichia coli in inclusion bodies, using a refolding system to obtain renatured proteins. Two types of refolded Fv were prepared: (i) Heavy and light chains of the immunoglobulin variable regions (VH and VL, respectively) were coexpressed with a dicistronic expression vector (designated Fv, co); (ii) VH and VL were expressed separately, mixed stoichiometrically, and refolded (designated Fvmix). All samples refolded with high efficiency; Fvco, Fvm;mix, scFv, and the bispecific diabody bound to several CEA-positive cell lines, exactly as did soluble Fv fragments secreted by E. coli (Fvsol) and the parent IgG. The refolded fragments inhibited binding of the parent IgG to CEA-positive cell lines, indicating that their epitope is identical to that of IgG. The bispecific diabody, which combined variable-region fragments of anti-CEA antibody with variable-region fragments of anti-CD3 antibody, was also prepared using the refolding system. This refolded diabody could bind to lymphokine-activated killer cells. In addition, its cytotoxicity toward human bile duct carcinoma TFK-1 and other several other CEA-positive cell lines was concentration-dependent. Taken together, our results suggest that a refolding procedure can be used to prepare various functional antibody fragments (Fv, scFv, and diabody).

• 社団法人 日本生化学会の論文

著者

• Tsumoto Kouhei

  Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University

• Makabe Koki

  Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University

• SUZUKI Masanori

  First Department of Surgery, Tohoku University School of Medicine

• Nishimura Yukihiro

  Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University

• Kumagai Izumi

  Department Of Biochemistry And Engineering Graduate School Of Engineering Tohoku University

• Kudo Toshio

  Cell Resource Center For Biomedical Research Institute Of Development Aging And Cancer Tohoku Univer

• Asano Ryutaro

  Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University

• Hayashi Hiroki

  Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University

• Kudo Toshio

  Cell Resource Center for Biomedical Research, Institute of Development, Aging, and Cancer, Tohoku University

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