Introduction of Enzymatic Activity to .ALPHA.-Lactalbumin by Artificial Exon Shuffling.
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概要
- 論文の詳細を見る
α-Lactalbumin and c-type lysozyme have quite different functions but their protein structures and gene organizations are very homologous. The exon 2 coding region of hen egg-white lysozyme contains most of amino acid residues comprising its active site cleft. We engineered a hybrid protein in which the exon 2 coding region of goat α-lactalbumin was replaced with that of hen lysozyme. This provided catalytic activity to α-lactalbumin.
- 日本学士院の論文
著者
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Takeda Shigeki
Department Of Chemistry And Chemical Biology Graduate School Of Engineering Gunma University
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Kumagai Izumi
Department Of Biochemistry And Engineering Graduate School Of Engineering Tohoku University
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Miura Kin-ichiro
Institute For Biomolecular Science Faculty Of Science Gakushuin University
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KUMAGAI Izumi
Department of Industrial Chemistry, Faculty of Engineering, The University of Tokyo
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