Rapid and Sensitive Detection of Brain-Derived Neurotrophic Factor with a Plasmonic Chip
スポンサーリンク
概要
- 論文の詳細を見る
Plasmonic chips, which are grating replicas coated with thin metal layers and overlayers such as ZnO, were applied in immunosensors to improve their detection sensitivity. Fluorescence from labeled antibodies bound to plasmonic chips can be enhanced on the basis of a grating-coupled surface plasmon resonance (GC-SPR) field. In this study, as one of the representative candidate protein markers for brain disorders, the brain-derived neurotrophic factor (BDNF) was quantitatively measured by sandwich assay on a plasmonic chip and detected on our plasmonic chip in the concentration of 5--7 ng/mL within 40 min. Furthermore, BDNF was detected in the blood sera from three types of mice: wild-type mice and two types of mutant mice. This technique is promising as a new clinical diagnosis tool for brain disorders based on scientific evidence such as blood test results.
- 2013-06-25
著者
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Kumagai Izumi
Department Of Biochemistry And Engineering Graduate School Of Engineering Tohoku University
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Umetsu Mitsuo
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Nakaoki Takahiko
Department Of Materials Chemistry Faculty Of Science And Technology Ryukoku University
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Tawa Keiko
Health Research Institute, AIST, Ikeda, Osaka 563-8577, Japan
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Aota Hiroyuki
Graduate School of Science and Engineering & ORDIST, Kansai University, Suita, Osaka 564-8680, Japan
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Uegaki Koichi
Health Research Institute, AIST, Ikeda, Osaka 563-8577, Japan
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Umetsu Mitsuo
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai 980-8579, Japan
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Kumagai Izumi
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai 980-8579, Japan
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Satoh Mari
Health Research Institute, AIST, Ikeda, Osaka 563-8577, Japan
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Hara Tomoko
Health Research Institute, AIST, Ikeda, Osaka 563-8577, Japan
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Kojima Masami
Health Research Institute, AIST, Ikeda, Osaka 563-8577, Japan
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Kumanogoh Haruko
Health Research Institute, AIST, Ikeda, Osaka 563-8577, Japan
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Yokota Yoshiki
Health Research Institute, AIST, Ikeda, Osaka 563-8577, Japan
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Nakazawa Hikaru
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai 980-8579, Japan
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Nakaoki Takahiko
Department of Material Science, Ryukoku University, Otsu 520-2194, Japan
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NAKAZAWA Hikaru
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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