Photochemically Induced Crystallization of Proteins Accelerated on Two-Dimensional Gold Gratings

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概要

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• The photochemically induced crystallization of lysozyme was studied on a two-dimensional surface relief grating coated with a thin gold film (2D-plasmonic chip), a flat thin gold film, and a flat UV-curable resin. The lysozyme solution prepared in a metastable compositional region was dropped on each surface. After an exposure to visible light from a filtered Hg lamp (500 and 800 nm in wavelength, 1.5 W/cm2) for 30 min followed by 18 h keeping in the dark, the crystal particles were observed in the droplets. Under a microscope, the numbers of crystalline particles were counted to be 1280, 258, and 180 on the 2D-plasmonic chip, the flat gold film, and the flat UV-curable resin, respectively. It was suggested that the crystallization tendency of lysozyme was effectively accelerated by the enhanced electric field caused by the coupling of incident light with surface plasmon polaritons on the 2D-plasmonic chip.

• 2012-06-25

著者

• Okutsu Tetsuo

  Graduate School Of Science And Engineering Department Of Chemistry Tokyo Institute Of Technology

• Tawa Keiko

  Health Research Institute, AIST, Ikeda, Osaka 563-8577, Japan

• Nishii Junji

  RIES, Hokkaido University, Sapporo 001-0021, Japan

• Haruta Susumu

  Graduate School of Engineering, Gunma University, Kiryu, Gunma 376-8515, Japan

• Okutsu Tetsuo

  Graduate School of Engineering, Gunma University, Kiryu, Gunma 376-8515, Japan

関連論文

• Chemically Induced Dynamic Electron Polarization in the Photoreduction of Aromatic Ketones with ππ^*-Type Triplet State
• Sensitive Fluorescence Microscopy of Neurons Cultured on a Plasmonic Chip
• Photochemically Induced Crystallization of Proteins Accelerated on Two-Dimensional Gold Gratings
• Rapid and Sensitive Detection of Brain-Derived Neurotrophic Factor with a Plasmonic Chip

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