P1-Specificity of Aqualysin I (a Subtilisin-type Serine Protease) from Thermus aquaticus YT-1,using P1-Substituted Derivatives of Streptomyces Subtilisin Inhibitor

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概要

• 論文の詳細を見る

• Aqualysin I is an alkaline serine protease isolated from Thermus aquaticus YT-1,an extreme thermophile. We have measured the P1-specificity of aqualysin I, using wild-type and five P1-substituted derivatives of Streptomyces subtilisin inhibitor (SSI). SSIs efficiently inhibited the activity of aqualysin I, with low substrate specificity. Charge and hydrophobicity of side chain of the P1 amino acid residue showed no significant effect to the P1-specificity of this enzyme.

• 社団法人日本農芸化学会の論文
• 1998-10-23

著者

• Ohta T

  Hokkaido Food Processing Res. Center Hokkaido Jpn

• Tanaka T

  Biofrontier Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Tanaka T

  Hiroshima Univ. Higashi Hiroshima Jpn

• Mori Hiroaki

  Department Of Agricultural Chemistry The University Of Tokyo

• Kumagai Izumi

  Department of Biomolecular Engineering, Tohoku University

• MATSUZAWA Hiroshi

  Department of Biotechnology, The University of Tokyo

• OHTA Takahisa

  Department of Agricultural Chemistry, The University of Tokyo

• Tanaka T

  Bio-organic Chemistry Laboratory Institute For Fundamental Research Suntory Research Center

• Tanaka Takaaki

  Graduate School Of Science And Technology Niigata University

• Kumagai Izumi

  Department Of Biochemistry And Engineering Graduate School Of Engineering Tohoku University

• Kumagai Izumi

  Department Of Industrial Chemistry Faculty Of Engineering The University Of Tokyo:(present Address)d

• Ohta Takahisa

  Department Of Agricultural Chemistry The University Of Tokyo

• Kojima S

  Gakushuin Univ. Tokyo Jpn

• Miura Kinichiro

  Department Of Industrial Chemistry Faculty Of Engineering The University Of Tokyo:(present Address)i

• Matsuzawa H

  Department Of Bioscience And Biotechnology Aomori University

• TANAKA Terumichi

  Department of Biotechnology, The University of Tokyo

• KOJIMA Shuichi

  Department of Industrial Chemistry, Faculty of Engineering, The University of Tokyo

• Koizumi S

  Institute For Biomolecular Science Gakushuin University

• Tanaka T

  Division Of Life Science And Biotechnology Department Of Ecological Engineering Toyohashi University

• Tanaka Takaaki

  Department Of Materials Science And Technology Faculty Of Engineering Niigata University

• Tanaka Terumichi

  Division Of Bioscience And Biotechnology Department Of Ecological Engineering Toyohashi University O

• Matsuzawa Hiroshi

  Department Of Biotechnology And Biotechnology Research Center The University Of Tokyo

• Matsuzawa Hiroshi

  Department of Bioscience and Biotechnology, Aomori University

• Kojima Shuichi

  Department of Applied Chemistry, Faculty of Technology, Kanagawa University

• MATSUZAWA Hiroshi

  Department of Agricultural Chemistry, The University of Tokyo

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