Substrate Specificity of Aqualysin I Altered by an Organic Solvent, DMSO

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概要

• 論文の詳細を見る

• Aqualysin I is the alkaline serine protease isolated from an extreme thermophile, Thermus aquaticus YT-1. We have analyzed the kinetic properties of aqualysin I, using thirty-one kinds of chromogenic succinyl-tripeptide p-nitroanilides as substrates in the presence of 10% dimethylsulfoxide (DMSO). Aqualysin I hydrolyzed many peptides in a DMSO-containing mixture, however the substrate specificity was different from that in the absence of DMSO. The K_m for each peptide was raised by the addition of 10% DMSO. Also, the P3-as well as P2-specificity of aqualysin I was altered. These results suggested that the side chains of the P2 and P3 residues are exposed to the solvent, and the hydrophobic interactions between the side chain of the substrate and the solvent may take a part in the substrate recognition of the enzyme.

• 社団法人日本農芸化学会の論文
• 1999-02-23

著者

• Ohta T

  Hokkaido Food Processing Res. Center Hokkaido Jpn

• Tanaka T

  Biofrontier Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Tanaka T

  Hiroshima Univ. Higashi Hiroshima Jpn

• Mori Hiroaki

  Department Of Agricultural Chemistry The University Of Tokyo

• MATSUZAWA Hiroshi

  Department of Biotechnology, The University of Tokyo

• OHTA Takahisa

  Department of Agricultural Chemistry, The University of Tokyo

• Tanaka T

  Bio-organic Chemistry Laboratory Institute For Fundamental Research Suntory Research Center

• Tanaka Takaaki

  Graduate School Of Science And Technology Niigata University

• Ohta Takahisa

  Department Of Agricultural Chemistry The University Of Tokyo

• Matsuzawa H

  Department Of Bioscience And Biotechnology Aomori University

• TANAKA Terumichi

  Department of Biotechnology, The University of Tokyo

• Tanaka T

  Division Of Life Science And Biotechnology Department Of Ecological Engineering Toyohashi University

• Tanaka Takaaki

  Department Of Materials Science And Technology Faculty Of Engineering Niigata University

• Tanaka Terumichi

  Division Of Bioscience And Biotechnology Department Of Ecological Engineering Toyohashi University O

• Matsuzawa Hiroshi

  Department Of Biotechnology And Biotechnology Research Center The University Of Tokyo

• Matsuzawa Hiroshi

  Department of Bioscience and Biotechnology, Aomori University

• MATSUZAWA Hiroshi

  Department of Agricultural Chemistry, The University of Tokyo

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