Isolation and Characterization of a Thermostable Aminopeptidase (Aminopeptidase T) from Thermus aquaticus YT-1, an Extremely Thermophilic Bacterium(Biological Chemistry)
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概要
- 論文の詳細を見る
A thermostable aminopeptidase, called aminopeptidase T, from the extract of Thermus aquaticus YT-1 was purified and characterized. The enzume had a dimeric structure, its relative molecular mass being 108,000 by gel filtration, and 48,000 by SDS-PAGE. The optimum pH of the enzyme activity was in the range of 8.5 to 9.0. The enzyme was singnificantly thermostable as it still retained 60% of its original activity even after heat treatment for 20 hr at 80℃. The enzyme activity was inhibited by metal-chelating agents. The enzyme had a low substrate specificity.
- 社団法人日本農芸化学会の論文
- 1988-07-23
著者
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山内 清
大阪市大 工
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Ohta T
Hokkaido Food Processing Res. Center Hokkaido Jpn
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Mori Hiroaki
Department Of Agricultural Chemistry The University Of Tokyo
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MATSUZAWA Hiroshi
Department of Biotechnology, The University of Tokyo
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Minagawa Etsuo
Department of Agricultural Chemistry, The University of Tokyo
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Ohta Takahisa
Department Of Agricultural Chemistry The University Of Tokyo
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Yamauchi K
Faculty Of Agriculture Miyazaki University
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Yamauchi Kunio
Department Of Agricultural Chemistry The University Of Tokyo
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Kaminogawa S
Dep. Of Food Sci. And Technol. Nihon Univ.
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MINAGAWA ETSUO
Research Center, Yotsuba Milk Products Co.Ltd.
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Minagawa Etsuo
Research Center Yotsuba Milk Products Co.ltd.
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Matsuzawa H
Department Of Bioscience And Biotechnology Aomori University
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Yamauchi K
Department Of Applied Chemistry & Bioengineering Graduate School Of Engineering Osaka City Unive
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Kaminogawa Shuichi
Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo
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Matsuzawa Hiroshi
Department Of Biotechnology And Biotechnology Research Center The University Of Tokyo
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Matsuzawa Hiroshi
Department of Bioscience and Biotechnology, Aomori University
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