Cloning and Nucleotide Sequencing of Genes Encoding Mn-Superoxide Dismutase and Class II Fumarase from Thermus aquaticus YT-1
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概要
- 論文の詳細を見る
A 6955-bp sequence of a PstI-HindIII DNA fragment containing the manganese-superoxide dismutase(MnSOD)gene of Thermus aquaticus YT-1 was determined. The gene(sod)encoded a polypeptide consisting of 204 amino acid residues(the mature enzyme without the initiation methionine)with a calculated Mr of 22, 773. The deduced amino acid sequence of the sod gene showed 92% identity to that of Thermus thermophilus HB8 determined chemically. The sod gene was well expressed in Escherichia coli and a heat-stable active enzyme was produced. An open reading frame encoding fumarase was found 91 bp upstream of the MnSOD gene in tandem form. The fum gene encoded a polypeptide consisting of 446 amino acid residues with a calculated M_r of 50, 950. The deduced amino acid swquence of the fum gene product has similarlity to that of the fumC of E.coli(57% identity), suggesting that this gene encodes a class II type fumarase.
- 社団法人日本生物工学会の論文
- 1998-07-25
著者
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Mori Hiroaki
Department Of Agricultural Chemistry The University Of Tokyo
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KAMINOGAWA Shuichi
Department of Food Science and Technology, College of Bioresource Sciences, Nihon University
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MOTOSHIMA Hidemasa
Research Center, Yotsuba Milk Products Co., Ltd.
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Kaminogawa Shuichi
Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Sciences Th
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Tsukasaki F
Research Center Yotsuba Milk Products Co. Ltd.
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Kaminogawa S
Dep. Of Food Sci. And Technol. Nihon Univ.
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TSUKASAKI Fuji
Research Center, Yotsuba Milk Products, Co., Ltd.
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MINAGAWA ETSUO
Research Center, Yotsuba Milk Products Co.Ltd.
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Minagawa Etsuo
Research Center Yotsuba Milk Products Co.ltd.
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Matsuzawa H
Department Of Bioscience And Biotechnology Aomori University
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Motoshima Hidemasa
Research Center Yotsuba Milk Products Co. Ltd.
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Kaminogawa Shuichi
Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo
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Tsukasaki Fuji
Research Center Yotsuba Milk Products Co. Ltd.
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Motoshima Hidemasa
Research and Development Department, Yotsuba Milk Products Co.
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