Characterization and Expression Analysis of the Exopolysaccharide Gene Cluster in Lactobacillus fermentum TDS030603
スポンサーリンク
概要
- 論文の詳細を見る
Part of the exopolysaccharide gene cluster of Lactobacillus fermentum TDS030603 was characterized. It consists of 11,890 base pairs and is located in the chromosomal DNA, 13 open reading frames of which were encoded. Out of the 13 open reading frames, six were found to be involved in exopolysaccharide synthesis; however, five were similar to transposase genes of other lactobacilli, and two were functionally unrelated. Expression analysis revealed that the exopolysaccharide synthesis-related genes were expressed during cultivation. Southern analysis using specific primers for the exopolysaccharide genes indicated that duplication of the gene cluster did not occur. The plasmid-cured strain maintained its capacity for exopolysaccharide production, confirming that the exopolysaccharide gene cluster of this strain is located in the chromosomal DNA, similarly to thermophilic lactic acid bacteria. Our results indicate that this exopolysaccharide gene cluster is likely to be functional, although extensive gene rearrangement occurs.
- 2009-12-23
著者
-
Takakuwa Naoya
Memuro Research Station, National Agricultural Research Center for Hokkaido Region
-
Urashima Tadasu
Meat And Milk Hygiene Graduate School Of Animal And Food Hygiene Obihiro Univ. Of Agriculture And Ve
-
Fukuda Kenji
Meat And Milk Hygiene Graduate School Of Animal And Food Hygiene Obihiro Univ. Of Agriculture And Ve
-
MOTOSHIMA Hidemasa
Research Center, Yotsuba Milk Products Co., Ltd.
-
DAN Tong
Meat and Milk Hygiene, Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture
-
SUGAI-BANNAI Michiko
Meat and Milk Hygiene, Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture
-
Dan Tong
Meat And Milk Hygiene Graduate School Of Animal And Food Hygiene Obihiro University Of Agriculture A
-
SUGAI BANNAI
Meat and Milk Hygiene, Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture
-
Sugai Bannai
Meat And Milk Hygiene Graduate School Of Animal And Food Hygiene Obihiro University Of Agriculture A
-
Motoshima Hidemasa
Research Center Yotsuba Milk Products Co. Ltd.
-
Takakuwa Naoya
Memuro Research Station National Agricultural Research Center For Hokkaido Region
-
Urashima Tadasu
Meat And Milk Hygiene Graduate School Of Animal And Food Hygiene Obihiro University Of Agriculture A
-
Fukuda Kenji
Meat And Milk Hygiene Graduate School Of Animal And Food Hygiene Obihiro University Of Agriculture A
-
Motoshima Hidemasa
Research and Development Department, Yotsuba Milk Products Co.
関連論文
- Characterization of Sterol Lipids in Kluyveromyces lactis Strain M-16 Accumulating a High Amount of Steryl Glucoside
- Thermostable β-Galactosidase from an Extreme Thermophile, Thermus sp. A4 : Enzyme Purification and Characterization, and Gene Cloning and Sequencing
- Structural determination of the oligosaccharides in the milk of a giant anteater (Myrmecophaga tridatyla)
- Purification and Characterization of a Novel Exo-β-1,3-1,6-glucanase from the Fruiting Body of the Edible Mushroom Enoki (Flammulina velutipes)
- Bovine Lactoferrin Stimulates Anchorage-Independent Cell Growth via Membrane-Associated Chondroitin Sulfate and Heparan Sulfate Proteoglycans in PC12 Cells
- Oral administration of freeze-dried kefir reduces intestinal permeation of and oral sensitization to ovalbumin in mice
- Screening for the Immunopotentiating Activity of Food Microorganisms and Enhancement of the Immune Response by Bifidobacterium adolescentis M101-4
- Characterization and Expression Analysis of the Exopolysaccharide Gene Cluster in Lactobacillus fermentum TDS030603
- Purification, Characterization, and Gene Cloning of Lysyl Aminoeptidase from Streptococcus thermophilus YRC001(Biochemistry & Molecular Biology)
- Cloning and Nucleotide Sequencing of Genes Encoding Mn-Superoxide Dismutase and Class II Fumarase from Thermus aquaticus YT-1
- Cloning of Genes of the Aminopeptidase T Family from Thermus thermophilus HB8 and Bacillus stearothermophilus NCIB8924 : Apparent Similarity to the Leucyl Aminopeptidase Family
- Content and Chemical Compositions of Cerebrosides in Lactose-assimilating Yeasts
- Effects of Growth Temperature on Cerebroside Content and Chemical Composition in Kluyveromyces lactis
- Characterization and Expression Analysis of the Exopolysaccharide Gene Cluster in Lactobacillus fermentum TDS030603
- Conversion of Beet Molasses and Cheese Whey into Fatty Acid Methyl Esters by the Yeast Cryptococcus curvatus
- Determination of Sialyl and Neutral Oligosaccharide Levels in Transition and Mature Milks of Samoan Women, Using Anthranilic Derivatization Followed by Reverse Phase High Performance Liquid Chromatography
- Purification and Characterization of a Novel Exo-β-1,3-1,6-glucanase from the Fruiting Body of the Edible Mushroom Enoki (Flammulina velutipes)
- Chemical characterization of milk oligosaccharides of an African lion (Panthera leo) and a clouded leopard (Neofelis nebulosa)
- Influence of Gelation Temperature on the Viscoelastic Properties of Glucono-δ-lactone-induced Milk Gel
- Influence of Gelation Temperature on the Viscoelastic Properties of Glucono-δ-lactone-induced Milk Gel
- Content and Constituent Properties of Sphingolipid Classes in Saccharomyces kluyveri
- Identification of Polypeptides Constituting Lactophorin by Monoclonal Antibody to Bovine Milk Lactophorin
- Changes in the bovine whey proteome during the early lactation period
- Chemical characterization of milk oligosaccharides of the island flying fox (Pteropus hypomelanus) (Chiroptera : Pteropodidae)
- Cloning, Monoclonal Antibody Production, and Bodily Distribution Pattern of a Bovine Lipocalin