Bovine Lactoferrin Stimulates Anchorage-Independent Cell Growth via Membrane-Associated Chondroitin Sulfate and Heparan Sulfate Proteoglycans in PC12 Cells
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概要
- 論文の詳細を見る
Bovine lactoferrin (bLf) is an iron-binding secretory protein present in breast milk, mucosal secretions, and the secondary granules of neutrophils. Although bLf has multiple functions, including antimicrobial and immunomodulatory activities, its effect on neuronal cells is not fully understood. We report that bLf prevents cell adhesion of PC12 cells and allows them to be cultivated in suspension. PC12 cells normally adhere well to plastic culture plates and show anchorage-dependent cell growth, but we found that soon after adding bLf, they detach from culture plates and begin to grow in suspension. When bLf was removed from the medium, the cells began to re-adhere to the plates. Thus, bLf inhibits cell adhesion and stimulates anchorage-independent growth in PC12 cells. On the other hand, bLf-induced cell suspension growth was not observed when cells were grown on a laminin matrix, suggesting that bLf does not affect integrin-mediated cell adhesion on a laminin matrix. Treatment of cells with heparin or chondroitin sulfate A or C inhibited bLf-induced growth in cell suspension. Furthermore, pretreatment of cells with heparinase and/or chondroitinase prevented direct binding of bLf to the cell membrane. These results suggest that bLf binds to the membrane of PC12 cells via membrane-associated proteoglycans and leads to anchorage-independent growth.
- 社団法人 日本薬理学会の論文
- 2007-08-20
著者
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Ishimori Hiroshi
Department of Cardiology, Saiseikai Yokohama City Eastern Hospital
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ISHII Toshiaki
Department of Molecular Physiology and Biochemistry, Research Institute for Advanced Science and Tec
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Fukuda Kenji
Graduate School of Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine
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URASHIMA Tadasu
Graduate School of Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine
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Ishii Toshiaki
岐阜大学 大学院連合獣医学研究科病態獣医学連合講座
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Ishii Toshiaki
Department Of Cardiology Surugadai-nihon University Hospital
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Ishii Toshiaki
Department Of Pathogenetic Veterinary Science The United Graduate School Of Veterinary Sciences Gifu
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Ishii Toshiaki
Department Of Pathobiological Science Obihiro University Of Agriculture And Veterinary Medicine
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Mori Kaori
Department Of Pathobiological Science Obihiro University Of Agriculture And Veterinary Medicine
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Urashima Tadasu
Meat And Milk Hygiene Graduate School Of Animal And Food Hygiene Obihiro Univ. Of Agriculture And Ve
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Urashima Tadasu
Graduate School Of Food Hygiene Obihiro University Of Agriculture And Veterinary Medicine
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Fukuda Kenji
Meat And Milk Hygiene Graduate School Of Animal And Food Hygiene Obihiro Univ. Of Agriculture And Ve
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UTO Tomoka
Department of Pathobiological Science, Obihiro University of Agriculture and Veterinary Medicine
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NISHIMURA Masakazu
Department of Pathobiological Science, Obihiro University of Agriculture and Veterinary Medicine
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Ishimori Hiroshi
Department Of Pathobiological Science Obihiro University Of Agriculture And Veterinary Medicine
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Ishii T
The Department Of Pulmonary Medicine Yokohama City University Medical Center
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Ishimori Hiroshi
Department Fo Cardiology Saiseikai Yokohama City Eastern Hospital
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Nishimura Masakazu
Department Of Pathobiological Science Obihiro University Of Agriculture And Veterinary Medicine
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Fukuda Kenji
Graduate School Of Food Hygiene Obihiro University Of Agriculture And Veterinary Medicine
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Uto Tomoka
Department Of Pathobiological Science Obihiro University Of Agriculture And Veterinary Medicine
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Fukuda Kenji
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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URASHIMA Tadasu
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine
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