Recombinant Adeno-associated Virus Vectors Efficiently Transduce Foreign Gene Into Bovine Aortic Endothelial Cells: Comparison With Adenovirus Vectors
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概要
- 論文の詳細を見る
Because the features and kinetics of adeno−associated virus(AAV)−mediated gene transfer to endothelial cells(EC)are yet to be ultimately determined, we tested variables pertinent to the efficiency of AAV−mediated gene transfer to bovine aortic endothelial cells(BAEC).The variables with AAV vectors were compared with the better characterized adenovirus(Ad)vectors.There is a dose−response relationship between multiplicity of infection(moi)of AAV or Ad vectors and transduction efficiency in BAEC.The higher moi of AAV vectors achieved more than 80% of transduction efficiency in cultured BAEC.AAV and Ad vectors showed an incubation−time−dependent increase in transduction efficiency of LacZ gene to the BAEC up to 12 h of vector exposure.Although the similar kinetics of transduction efficiency of LacZ gene to BAEC was found in both vectors, the duration of gene expression was longer in AAV vector than that in Ad vectors in vitro.These results indicate that AAV−vector is efficient for gene transfer to EC, and higher moi of vectors or a longer period exposure of vectors to EC can facilitate efficient transduction of a foreign gene into cultured EC.For the duration of gene expression, the AAV vectors may be better than Ad vectors.
- 社団法人 日本薬理学会の論文
- 2000-10-01
著者
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FUKUCHI Yoshinosuke
Department of Respiratory Medicine Juntendo University, School of Medicine
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松瀬 健
The Department Of Pulmonary Medicine Yokohama City University Medical Center
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MATSUSE Takeshi
Department of Geriatric Medicine, University of Tokyo Hospital
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Ishii Toshiaki
岐阜大学 大学院連合獣医学研究科病態獣医学連合講座
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Ishii Toshiaki
Department Of Pathogenetic Veterinary Science The United Graduate School Of Veterinary Sciences Gifu
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Ishii Toshiaki
Department Of Pathobiological Science Obihiro University Of Agriculture And Veterinary Medicine
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TERAMOTO Shinji
Department of Internal Medicine, San-no Hospital
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ISHII Takeo
Department of Geriatric Medicine, Tokyo University Hospital
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Teramoto Shinji
The Department Of Geriatric Medicine The University Of Tokyo Hospital
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Teramoto Shinji
Department Of Geriatric Medicine Graduate School Of Medicine The University Of Tokyo
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Ishii T
The Department Of Pulmonary Medicine Yokohama City University Medical Center
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Matsuse Takeshi
Department Of Geriatric Medicine University Of Tokyo Hospital
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Fukuchi Yoshinosuke
Deparcment Of Geriacrics Tokvo University
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TERAMOTO Shinji
National Hospital Organization Tokyo Hospital
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Fukuchi Y
Department Of Respiratory Medicine Juntendo University School Of Medicine
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