Effect of Ambroxol on Oxygen Radical Production and Generation by Bronchoalveolar Lavage Cells in Young and Aged Guinea Pigs
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概要
- 論文の詳細を見る
We examined the effect of ambroxol and age on oxygen radical production and generation with stimulation of phorbol-myristate acetate (PMA) by bronchoalveolar lavage (BAL) cells. Lung free cells including pulmonary alveolar macrophages were harvested from young (4-month-old) and aged (28-month-old) male guinea pigs using BAL. The oxygen radicals produced by BAL cells with or without stimulation of PMA were measured by the lucigenin-dependent chemiluminescence method using a photon counter. Oxygen radical production and generation by BAL cells were not different between young and aged guinea pigs. However, the oxygen radical generation after stimulation with PMA was greater than the oxygen radical spontaneous production both in young and aged animals. Ambroxol solution given into culture media containing BAL cells inhibited oxygen radical production and generation by BAL cells harvested from both young and aged guinea pigs in a concentration-dependent manner. Approximately 16 - 20 μM of ambroxol inhibited 50% of the production of oxygen radicals in vitro by BAL cells in young and aged guinea pigs, whereas a slightly greater amount of ambroxol was necessary to inhibit 50% of the PMA-induced oxygen radical generation in vitro by BAL cells in guinea pigs. These results indicate that ambroxol inhibits oxygen radicals produced by BAL cells from young and aged guinea pigs, and they suggest that ambroxol may be a possible therapeutic modality for ameliorating oxidant associated pulmonary disorders in young and aged patients.
- 社団法人 日本薬理学会の論文
- 1998-12-01
著者
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OUCHI Yasuyoshi
Department of Geriatric Medicine, University of Tokyo
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松瀬 健
The Department Of Pulmonary Medicine Yokohama City University Medical Center
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MATSUSE Takeshi
Department of Geriatric Medicine, University of Tokyo Hospital
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TERAMOTO Shinji
Department of Internal Medicine, San-no Hospital
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SUZUKI Masashi
Department of Geriatric Medicine, Tokyo University Hospital
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Teramoto Shinji
The Department Of Geriatric Medicine The University Of Tokyo Hospital
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Teramoto Shinji
Department Of Geriatric Medicine Graduate School Of Medicine The University Of Tokyo
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Ouchi Yasuyoshi
Department Of Geriatric Medicine University Of Tokyo Hospital
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Ouchi Yasuyoshi
Department Of Geriatric Medicine Faculty Of Medicine The University Of Tokyo
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Matsuse Takeshi
Department Of Geriatric Medicine University Of Tokyo Hospital
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TERAMOTO Shinji
National Hospital Organization Tokyo Hospital
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Suzuki Masashi
Department Of Geriatric Medicine Tokyo University Hospital
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Suzuki Masashi
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Ouchi Yasuyoshi
Department Of Biostatistics Epidemiology And Preventive Health Sciences University Of Tokyo
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Ouchi Yasuyoshi
Department Of Biostatistics Epidemiology And Preventive Health Sciences The University Of Tokyo
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