Characterization of Muscarinic Receptor-Mediated Cationic Currents in Longitudinal Smooth Muscle Cells of Mouse Small Intestine
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概要
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In mouse intestinal smooth muscle cells held at −50 mV, carbachol evoked an atropine-sensitive inward current in the intracellular presence of Cs+. The current response consisted of an initial peak followed by a smaller plateau component on which oscillatory currents frequently arose. Results from various experimental procedures indicated that the inward current is a muscarinic receptor-operated cationic current (mIcat) sensitive to cytosolic Ca2+ concentration ([Ca2+]i) and that the initial peak and oscillatory components are contaminated by Ca2+-activated Cl− currents. Under conditions of [Ca2+]i buffered to 100 nM, the mIcat response to cumulative carbachol applications was inhibited competitively by an M2-selective antagonist but non-competitively by an M3-selective one. Also it was severely reduced by pertussis toxin (PTX) treatment or a phospholipase C (PLC) inhibitor. Comparative analysis of mIcat in mouse and guinea-pig intestinal myocytes indicated that the underlying channels resemble between those myocytes in agonist sensitivity, current-voltage relationship, and unitary conductance. The results suggest that in mouse intestinal myocytes, mIcat arises mainly via an M2/M3 synergistic mechanism involving PTX-sensitive G-proteins and PLC activity in the absence of current modulation by [Ca2+]i changes, as described for guinea-pig ileal mIcat. The channels underlying mIcat are also indistinguishable in gating properties between both types of myocytes.
- 2006-03-20
著者
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Sakamoto Takashi
Department of Cardiology, Kyoto prefectural Yosanoumi Hospital
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MATSUYAMA Hayato
Laboratory of Veterinary Pharmacology, Department of Veterinary Medicine, Faculty of Applied Biologi
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UNNO Toshihiro
Laboratory of Veterinary Pharmacology, Department of Veterinary Medicine, Faculty of Applied Biologi
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KOMORI Seiichi
Laboratory of Veterinary Pharmacology, Department of Veterinary Medicine, Faculty of Applied Biologi
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Komori Seiichi
Department Of Pathogenetic Veterinary Science The United Graduate School Of Veterinary Sciences Gifu
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Komori S
Gifu Univ. Gifu Jpn
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Unno T
Gifu Univ. Gifu Jpn
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Unno Toshihiro
Department Of Pathogenic Veterinary Science United Graduate School Of Veterinary Science Gifu Univer
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NISHIMURA Masakazu
Department of Pathobiological Science, Obihiro University of Agriculture and Veterinary Medicine
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Komori Seiichi
Laboratory Of Pharmacology Department Of Veterinary Medicine Faculty Of Agriculture United Graduate
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Matsuyama Hayato
Laboratory Of Pharmacology Department Of Veterinary Medicine Faculty Of Applied Biological Science G
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Unno Toshihiro
Laboratory Of Pharmacology Department Of Veterinary Medicine Faculty Of Agriculture United Graduate
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Nishimura M
Department Of Pathobiological Science Obihiro University Of Agriculture And Veterinary Medicine
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UCHIYAMA Mai
Laboratory of Pharmacology, Department of Veterinary Medicine, Faculty of Applied Biological Science
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HATTORI Mitsunobu
Laboratory of Pharmacology, Department of Veterinary Medicine, Faculty of Applied Biological Science
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Uchiyama Mai
Laboratory Of Pharmacology Department Of Veterinary Medicine Faculty Of Applied Biological Science G
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Nishimura Masakazu
Department Of Pathobiological Science Obihiro University Of Agriculture And Veterinary Medicine
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Sakamoto Takashi
Department Of Cardiology Kyoto First Red Cross Hospital
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Hattori Mitsunobu
Laboratory Of Pharmacology Department Of Veterinary Medicine Faculty Of Applied Biological Science G
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Sakamoto Takashi
Department Of Aquatic Biosciences Tokyo University Of Fisheries
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Sakamoto Takashi
Department Of Pathogenic Veterinary Science United Graduate School Of Veterinary Science Gifu Univer
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