Smooth muscle excitatory substances from Remak nerve of the chicken and a comparison of their pharmacological and chemical properties with substance P.
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概要
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Active substances extracted from the Remak nerve of the chicken were subjected to chromatographic and electrophoretic separation followed by bioassay of contracting activities on the longitudinal muscle of the guinea-pig ileum (LMGPI) and on the isolated whole chick rectum (WCR). Gel filtration profiles on a Sephadex G-50 column showed two peaks of LMGPI-contracting activity and of WCR-contracting activity. No difference was seen in the enzymatic destruction between the LMGPI-contracting activity and substance P. Their similarities were also indicated by the parallelism of their elution curves in the gel chromatography on Sephadex G-25, their equal stability in acid solutions, and comparable antagonism and inhibition of the contractile effects on LMGPI by substance P antagonists and after desensitization of substance P receptors. Ion exchange chromatography revealed the existence of two main substances responsible for the LMGPI-contracting activity. One of them eluted at the same position as that for substance P, but differed in immunoreactivity and electrophoretic mobility from substance P. The WCR-contracting activity differed from the LMGPI-contracting activity in that it was pepsin-resistant and carboxypeptidase A-susceptible, and it eluted at a different position during ion exchange chromatography. It seems likely that the LMGPI-contracting activity in the extracts is attributed to a substance P-family of peptides, but the WCR-contracting activity is due to another substance of a peptide nature.
- 公益社団法人 日本薬理学会の論文
著者
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Komori Seiichi
Laboratory Of Pharmacology Department Of Veterinary Medicine Faculty Of Agriculture United Graduate
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Ohashi Hidenori
Laboratory Of Pharmacology Department Of Veterinary Medicine Faculty Of Agriculture United Graduate
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KANAMARU Yoshihiro
Laboratory of Animal Products Technology, Department of Poultry and Animal Science, Faculty of Agriculture, Gifu University
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MATSUO Konomi
Laboratory of Pharmacology, Department of Veterinary Medicine, Faculty of Agriculture, Gifu University
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