Localization of Sequential Antigenic Determinants on Bovine β-Casein with Synthetic Peptides and Antisera from Mouse, Rabbit, and Goat
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概要
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The antigenic determinants of bovine β-casein (β-CN) were localized by using twenty overlapping peptides encompassing the entire sequence of β-CN and anti-β-CN antisera from outbred mouse, rabbit and goat. The profile of the reactions was characteristic to the species, the dominant antigenic regions being 80-95,143-158 and 195-209 in mouse, 1-16 in rabbit and 100-115 in goat. Regions 1-16,100-115,121-136 and 143-158 were antigenic in all three species. The number of antigenic regions recognized by goat was much fewer than that by mouse and rabbit, possibly because of the homology between bovine and goat β-CN. A mixture of the twenty peptides could absorb about 50-60% of β-CN specific antibodies from each species. Furthermore, the mouse and rabbit anti-β-CN antibodies were also specific to the phosphorylated regions. We therefore conclude that the major antigenic determinants on β-CN would be largely sequential and include the phosphorylated sites.
- 社団法人日本農芸化学会の論文
- 1999-05-23
著者
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Mizumachi K
National Inst. Of Livestock And Grassland Sci. Ibaraki Jpn
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Mizumachi Koko
Department Of Animal Products National Institute Of Livestock And Grassland Science
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Kaminogawa Shuichi
Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo
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KURISAKI Junichi
Department of Animal Products, National Institute of Animal Industry
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Kurisaki J
Department Of Animal Products National Institute Of Animal Industry
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KURISAKI Junichi
Department of Agricultural Chemistry, The University of Tokyo
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