Signal Peptide of the Colicin E2 Lysis Protein Causes Host Cell Death(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
Colicin E2 is encoded by the SOS-inducible colicin operon of plasmid ColE2-P9. Highly induced colicin E2 is excreted from the E. coli host cell, accompanied by quasi-lysis and cell death. These three events are all directed by the colicin lysis protein that comprises the signal peptide and a small mature lipoprotein. We constructed a mutant lysis gene encoding its complete signal peptide with addition of two external amino acids at the C-terminus. By using this mutant peptide as a reference, the wild type lysis signal peptide was identified and shown to remain quite stable after the cleavage. This mutant peptide also mimics the cleaved-off signal peptide avoiding the influence of the mature lysis lipoprotein, and suggested that cell death by the lysis protein, but neither colicin excretion nor quasi-lysis, is decided by the nature of its signal region.
- 社団法人日本農芸化学会の論文
- 1991-06-23
著者
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MASAKI Haruhiko
Department of Biotechnology, The University of Tokyo
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OHTA Takahisa
Department of Agricultural Chemistry, The University of Tokyo
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UOZUMI Takeshi
Department of Biotechnology, Faculty of Agriculture, The University of Tokyo
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Masaki Haruhiko
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Ohta Takahisa
Department Of Agricultural Chemistry The University Of Tokyo
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Ohta Takahisa
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Uozumi Takeshi
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Uozumi Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Kanoh Shingo
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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YAJIMA Shunsuke
Department of Biotechnology, Faculty of Agriculture, The University of Tokyo
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Yajima Shunsuke
Department Of Bioscience Tokyo University Of Agriculture
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Yajima Shunsuke
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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