Mutational Analysis of the Amino Acid Residues Essential for the cis and trans Cleavage Activity of the Potato Virus Y 50-kDa Protease
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概要
- 論文の詳細を見る
To examine the proteolytic activities of various truncated derivatives of the potato virus Y (PVY)50-kDa protease, the derivatives were expressed in Escherichia coli in polyprotein forms fused with coat protein (CP). For the intermolecular cleavage reaction, the truncated proteases were expressed together with the substrate protein containing the polymerase-CP junction. The activity was evaluated by the amount of the mature CP released from the precursor by the intra- and intermolecular cleavage occurring in E. coli. By this experiment, we identified the moiety responsible for the proteolytic activity of the 50-kDa protease to be a 26-kDa polypeptide mapped to the C-terminal half of the protease. Introduction of His234→Tyr, Asp269→Asn, or Cys339→Gly substitution in the putative catalytic triad of the protease abolished its activity. However, the mutated protease with Cys339→Ser replacement retained a reduced proteolytic activity.
- 社団法人日本農芸化学会の論文
- 1993-09-23
著者
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HIDAKA Makoto
Department of Biotechnology, The University of Tokyo
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MASAKI Haruhiko
Department of Biotechnology, The University of Tokyo
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Hidaka Makoto
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Uozumi T
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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YAMAOKA Yukiho
Government Industrial Research Institute
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UOZUMI Takeshi
Department of Biotechnology, Faculty of Agriculture, The University of Tokyo
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Masaki Haruhiko
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Yoshida Y
Government Industrial Research Institute
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Uozumi Takeshi
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Uozumi Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Yoshida Yukiko
Department Of Chemical Pharmacology Toho University School Of Pharmaceutical Sciences
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Masaki H
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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