Molecular Cloning of a Cellulase Gene from Bacillus subtills and Its Expression in Escherichia coli
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概要
- 論文の詳細を見る
The gene for an endolytic cellulase of Bacillus subtilis was cloned on pBD64 in B. subtilis. The recombinant plasmid, pBC5, contained a 4.8-kb fragment derived from the B. subtilis chromosome, and the transformant overproduced the extracellular enzyme. The inserted fragment was subcloned on pBR322 and the recombinant plasmid, pBRC5, was introduced into Escherichia coli JM105. The E. coli transformant produced the enzyme in both the periplasmic space of cells and the cultural broth.
- 社団法人 日本農芸化学会の論文
著者
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Uozumi Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Beppu Teruhiko
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Nakamura Akira
Department Oc Macromolecular Science Faculty Of Science Osaka University
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KOIDE Yoshinao
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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