Characterization of the Leader Peptide of an Endo-type Cellulase Produced by an Alkalophilic Streptomyces Strain(Microbiology & Fermentation Industry)

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概要

• 論文の詳細を見る

• An endo-type semi-alkaline cellulase (CMCase I) produced by an alkalophilic Streptomyces strain has an extraordinarily long leader peptide of about 70 amino acids (aa), which can be grouped into four distinct regions, an NH2-terminal region (13aa), an Arg-cluster region (13aa), a hydrophobic region (23aa), and an Ala/Pro-repeat region (12aa). For identification of the function of each part of the leader peptide for secretion of the enzyme, mutations in the leader peptide were generated by site-directed mutagenesis using the cloned gene, and the mutant genes were expressed in a cellulase-negative mutant strain, Streptomyces lividans HN1. Although all the alterations in the leader peptide, except for one case, decreased secretion to various extents, in S. lividans, the following conclusions were obtained. Comparison of the intra- and extra-cellular enzyme activities of the mutants suggested that the Arg-cluster region was essential in secretion of the cellulase. Deletion of 8 amino acids rich in threonine and proline in the NH_2-terminal region enhanced the secretion to a small extent. Deletion of the Ala/Pro repeat region had almost no effect on secretion.

• 社団法人日本農芸化学会の論文
• 1991-07-23

著者

• HORINOUCHI Sueharu

  Department of Biotechnology, University of Tokyo

• BEPPU Teruhiko

  Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tok

• Horinouchi Sueharu

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Horinouchi Sueharu

  Dep. Of Biotechnology Graduate School Of Agriculture And Life Sciences Univ. Of Tokyo

• Park J‐s

  Dr. Chung's Food Co. Ltd. Choongchungbuk‐do Kor

• Beppu T

  Department Of Applied Biological Science Nihon University

• Park Jae-Seon

  Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo

• Beppu Teruhiko

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

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