Conversion of a Cyanhydrin Compound into S-(-)-3-Phenyllactic Acid by Enantioselective Hydrolytic Activity of Pseudomonas sp. BC-18

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A Pseudomonas strain, named BC-18, which can convert racemic phenylacetaldehyde-cyanhydrin (3-phenyllactonitrile) enantioselectively to S-(-)-3-phenyllactic acid (S-PLA), was isolated from soil. Although PLA produced with intact cells contained the S enantiomers of approximately 75% enantiomeric excess (% e.e.), repeated crystallization gave a higher purify (99.8% e.e) of the S configuration product. Production of S-PLA was significantly increased when 2.0% (w/v) of calcium chloride were added to the reaction mixture for precipitation of S-PLA. Chemical mutagenesis yielded a mutant strain, named BC348-9, with 16 times higher activity (40mU/OD^<630>), compared with that of the parent strain (2.5mU/OD^<630>). When the mutant strain BC348-9 was used, approximately 18 g/OD^<630> was produced, which is 12 times higher than that of the parent strain. The final accumulation of PLA exceeded 6.0%, 1.2 times higher than that of the parent strain.
社団法人日本農芸化学会の論文
1996-08-23