Transglycosylation Activity of Dictyoglomus thermophilum Amylase A
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概要
- 論文の詳細を見る
Amylase A from Dictyoglomus thermophilum is a thermophilic enzyme and has about 40% identity with 4-α-glucanotransferase (GTase) from Thermococcus litoralis, and both of these enzymes belong to family 57 glycosyl hydrolase. Since the transglycosylation activity of T. litoralis GTase has been well characterized, the substrate specificity and reaction products of amylase A from D. thermophilum were examined. α-1,4 Glucan was produced from maltooligosaccharides, and glucoamylase-resistant molecules (cycloamyloses) were produced from longer chain amylose (average molecular mass 200 kDa). It has been reported that amylase A from D. thermophilum hydrolyzes starch, but in this study it was found that the enzyme was also able to use maltooligosaccharides and long chain amylose as substrate and has transglycosylation activity.
- 社団法人 日本農芸化学会の論文
- 2004-11-23
著者
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WAKAGI Takayoshi
Department of Biotechnology, The University of Tokyo
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SHOUN Hirofumi
Department of Biotechnology, The University of Tokyo
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Wakagi Takayoshi
Department Of Biotechnology University Of Tokyo
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NAKAJIMA Masahiro
Department of Biotechnology, University of Tokyo
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IMAMURA Hiromi
Department of Biotechnology, University of Tokyo
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HORINOUCHI Sueharu
Department of Biotechnology, University of Tokyo
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Imamura Hiromi
Department Of Biotechnology University Of Tokyo
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Shoun Hirofumi
Department Of Biotechnology University Of Tokyo
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Shoun Hirofumi
Department Of Agricultural Chemistry The University Of Tokyo:(present Office)institute Of Applied Bi
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Horinouchi Sueharu
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Horinouchi Sueharu
Department Of Biotechnology University Of Tokyo
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Nakajima Masahiro
Department Of Biotechnology University Of Tokyo
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Wakagi Takayoshi
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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