A Eukaryotic Copper-Containing Nitrite Reductase Derived from a NirK Homolog Gene of Aspergillus oryzae
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概要
- 論文の詳細を見る
We cloned a bacterial copper-containing nitrite reductase (NirK) homolog gene of Aspergillus oryzae (AonirK). Alignment showed that amino acid residues crucial for copper binding are conserved in the deduced sequence of the fungal protein. The recombinant protein exhibited distinct nitrite reductase activity, and its absorption and EPR spectra showed the presence of type 1 and type 2 copper atoms in the molecule. AonirK transcriptionally responded to denitrification conditions. Although the denitrifying activity of A. oryzae was weak under the conditions employed, high expression of the gene in the fungal cells enhanced the denitrifying activity 6-fold, accompanied by distinct cell growth. Furthermore, the highly expressed AoNirK was subcellularly localized to the mitochondria. The results demonstrated that AoNirK is responsible for fungal denitrification. Discussion is added on the novel insight concerning the origin and evolution of the mitochondrion provided by the findings for eukaryotic NirKs.
- 社団法人 日本農芸化学会の論文
- 2010-05-23
著者
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Kitamoto Katsuhiko
Department of Biotechnology, The University of Tokyo
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Fushinobu Shinya
Department of Biotechnology, The University of Tokyo
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WAKAGI Takayoshi
Department of Biotechnology, The University of Tokyo
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SHOUN Hirofumi
Department of Biotechnology, The University of Tokyo
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Shoun Hirofumi
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Shoun Hirofumi
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Fushinobu Shinya
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Fushinobu Shinya
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Wakagi Takayoshi
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Wakagi T
Univ. Tokyo Tokyo Jpn
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Kim Sang-Wan
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of To
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Zhou Shengmin
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of To
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MARUYAMA Jun-ichi
Department of Biotechnology, The University of Tokyo
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Shoun Hirofumi
Department Of Agricultural Chemistry The University Of Tokyo:(present Office)institute Of Applied Bi
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Nakanishi Yoshito
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of To
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Kitamoto Katsuhiko
Department Of Applied Biological Chemistry And Department Of Applied Biological Engineering Graduate
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Kitamoto Katsuhiko
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Nakanishi Yoshito
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Kim Sang-wan
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Maruyama Jun-ichi
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Wakagi Takayoshi
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Zhou Shengmin
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Fushinobu Shinya
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Shoun Hirofumi
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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