The Role of Conserved Arginine Residue in Loop 4 of Glycoside Hydrolase Family 10 Xylanases
スポンサーリンク
概要
- 論文の詳細を見る
An arginine residue in loop 4 connecting β strand 4 and α-helix 4 is conserved in glycoside hydrolase family 10 (GH10) xylanases. The arginine residues, Arg204 in xylanase A from Bacillus halodurans C-125 (XynA) and Arg196 in xylanase B from Clostridium stercorarium F9 (XynB), were replaced by glutamic acid, lysine, or glutamine residues (XynA R204E, K and Q, and XynB R196E, K and Q). The pH-kcat⁄Km and the pH-kcat relationships of these mutant enzymes were measured. The pKe2 and pKes2 values calculated from these curves were 8.59 and 8.29 (R204E), 8.59 and 8.10 (R204K), 8.61 and 8.19 (R204Q), 7.42 and 7.19 (R196E), 7.49 and 7.18 (R196K), and 7.86 and 7.38 (R196Q) respectively. Only the pKes2 value of arginine derivatives was less than those of the wild types (8.49 and 9.39 [XynA] and 7.62 and 7.82 [XynB]). These results suggest that the conserved arginine residue in GH10 xylanases increases the pKa value of the proton donor Glu during substrate binding. The arginine residue is considered to clamp the proton donor and subsite +1 to prevent structural change during substrate binding.
- 2005-05-23
著者
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Kitaoka Motomitsu
National Food Research Institute, National Agriculture and Food Research Organization
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Fushinobu Shinya
Department of Biotechnology, The University of Tokyo
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Fushinobu Shinya
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Kitaoka M
Enzyme Laboratory National Food Research Institute
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Kitaoka M
Chubu Univ. Aichi Jpn
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Kitaoka Motomitsu
National Food Res. Inst.
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Kitaoka Motomitsu
Enzyme Laboratory National Food Research Institute National Agriculture And Food Research Organizati
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Miyatake K
Osaka Prefecture Univ. Osaka Jpn
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Hayashi K
Enzyme Laboratory National Food Research Institute
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Hayashi Kiyoshi
National Food Research Institute Ministry Of Agriculture Forestry And Fisheries
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Nishimoto Mamoru
National Food Research Institute
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切畑 光統
大阪府立大学 農学部
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Nishimoto Mamoru
Enzyme Laboratory National Food Research Institute
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Nishimoto Mamoru
National Food Res. Inst.
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Hayashi Kiyoshi
National Food Research Institute
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Nishimoto Mamoru
Enzyme Laboratory National Food Research Institute National Agriculture And Food Research Organizati
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Kitaoka Motomitsu
National Agriculture And Food Research Organization National Food Research Institute
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Nishimoto Mamoru
National Agriculture And Food Research Organization National Food Research Institute
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