Practical Preparation of Lacto-N-biose I, a Candidate for the Bifidus Factor in Human Milk
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概要
- 論文の詳細を見る
A one-pot enzymatic reaction to produce lacto-N-biose I (LNB), which is supposed to represent the bifidus factor in human milk oligosaccharides, was demonstrated. Approximately 500 mM of LNB was generated in 10-liter of reaction mixture initially containing 660 mM of sucrose and 600 mM of GlcNAc by the concurrent actions of four enzymes, sucrose phosphorylase, UDP-glucose—hexose-1-phospate uridylyltransferase, UDP-glucose 4-epimerase, and lacto-N-biose phosphorylase, in the presence of UDP-Glc and phosphate, indicating a reaction yield of 83%. LNB was isolated from the mixture by crystallization after yeast treatment. Finally, 1.4 kg of LNB of 99.6% purity was recovered after recrystallization.
- 社団法人 日本農芸化学会の論文
- 2007-08-23
著者
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Kitaoka Motomitsu
National Food Res. Inst.
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Nishimoto Mamoru
National Food Res. Inst.
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Kitaoka Motomitsu
National Agriculture And Food Research Organization National Food Research Institute
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Nishimoto Mamoru
National Agriculture And Food Research Organization National Food Research Institute
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