Diversity and Similarity of Microbial Communities in Petroleum Crude Oils Produced in Asia
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概要
- 論文の詳細を見る
To understand microbial communities in petroleum crude oils, we precipitated DNA using high concentrations of 2,2,4-trimethylpentane (isooctane) and purified. Samples of DNA from five crude oils, (Middle East, 3; China, 1; and Japan, 1) were characterized based upon their 16S rRNA gene sequences after PCR amplification and the construction of clone libraries. We detected 48 eubacterial species, one cyanobacterium, and one archaeon in total. The microbial constituents were diverse in the DNA samples. Most of the bacteria affiliated with the sequences of the three oils from the Middle East comprised similar mesophilic species. Acinetobacter, Propionibacterium, Sphingobium and a Bacillales were common. In contrast, the bacterial communities in Japanese and Chinese samples were unique. Thermophilic Petrotoga-like bacteria (11%) and several anaerobic-thermophilic Clostridia- and Synergistetes-like bacteria (20%) were detected in the Chinese sample. Different thermophiles (12%) and Clostridia (2%) were detected in the Japanese sample.
- 社団法人 日本農芸化学会の論文
- 2008-11-23
著者
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Uchiyama Hiroo
Graduate School of Life and Environmental Sciences, University of Tsukuba
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Nomura Nobuhiko
Graduate School of Life and Environmental Sciences, University of Tsukuba
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Kitaoka Motomitsu
National Food Res. Inst.
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Nakajima-kambe Toshiaki
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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MAKI HIDEAKI
National Institute for Environmental Studies
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Uchiyama Hiroo
Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
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Uchiyama Hiroo
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Nomura Nobuhiko
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Nomura Nobuhiko
Graduate School Of Life And Environmental Science University Of Tsukuba
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Nakajima-kambe Toshiaki
Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
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Yamane Kunio
National Food Research Institute
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Nakayama Tsuyoshi
Graduate School of Life and Environmental Sciences, University of Tsukuba
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NAKAJIMA Toshiaki
Graduate School of Life and Environmental Sciences, University of Tsukuba
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Nakae T
Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
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Nakajima Toshiaki
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Kitaoka Motomitsu
National Agriculture And Food Research Organization National Food Research Institute
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