Characterization of Glycosynthase Mutants Derived from Glycoside Hydrolase Family 10 Xylanases
スポンサーリンク
概要
- 論文の詳細を見る
Four xylanases belonging to glycoside hydrolase family 10—Thermotoga maritima XylB (TM), Clostridium stercorarium XynB (CS), Bacillus halodurans XynA (BH), and Cellulomonas fimi Cex (CF)—were converted to glycosynthases by substituting the nucleophilic glutamic acid residues with glycine, alanine, and serine. The glycine mutants exhibited the highest levels of glycosynthase activity with all four enzymes. All the glycine mutants formed polymeric β-1,4-linked xylopyranose as a precipitate during reaction with α-xylobiosyl fluoride. Two glycine mutants (TM and CF) recognized X2 as an effective acceptor molecule to prohibit the formation of the polymer, while the other two (CS and BH) did not. The difference in acceptor specificity is considered to reflect the difference in substrate affinity at their +2 subsites. The results agreed with the structural predictions of the subsite, where TM and CF exhibit high affinity at subsite 2, suggesting that the glycosynthase technique is useful for investigating the affinity of +subsites.
- 社団法人 日本農芸化学会の論文
- 2006-05-23
著者
-
Kitaoka Motomitsu
National Food Res. Inst.
-
Kitaoka Motomitsu
Enzyme Laboratory National Food Research Institute
-
Nishimoto Mamoru
Enzyme Laboratory National Food Research Institute
-
SUGIMURA Masahiro
Enzyme Laboratory, National Food Research Institute
-
Sugimura Masahiro
Enzyme Laboratory National Food Research Institute
関連論文
- The Role of Conserved Arginine Residue in Loop 4 of Glycoside Hydrolase Family 10 Xylanases
- Crystallographic and Mutational Analyses of Substrate Recognition of Endo-α-N-acetylgalactosaminidase from Bifidobacterium longum
- Kinetic Studies on p-Nitrophenyl-cellobioside Hydrolyzing Xylanase from Cellvibrio gilvus
- 647 A putative proline iminopeptidase of Thermotoga maritime with leucine- and lysine-p-nitroanilide hydrolyzing activities
- Characterization of a Cellobiose Phosphorylase from a Hyperthermophilic Eubacterium, Thermotoga maritima MSB8(Biochemistry & Molecular Biology)
- Kinetic Studies of a Recombinant Cellobiose Phosphorylase (CBP) of the Clostridium thermocellum YM4 Strain Expressed in Escherichia coli
- Effects of Truncation at the Non-homologous Region of a Family 3 β-Glucosidase from Agrobacterium tumefaciens
- A Thermostable Non-xylanolytic α-Glucuronidase of Thermotoga maritima MSB8(Biochemistry & Molecular Biology)
- Syntheses of 4-Methylumbelliferyl-β-D-Xylobioside and 5-Bromo-3-Indolyl-β-D-Xylobioside for Sensitive Detection of Xylanase Activity on Agar Plates
- Characterization of a Thermostable Family 10 Endo-Xylanase (XynB) from Thermotoga maritima That Cleaves p-Nitrophenyl-β-D-Xlyloside
- Analyses of Bifidobacterial Glycosidases Involved in the Metabolism of Oligosaccharides
- 糖質加リン酸分解酵素
- Kinetic Studies on the Hydrolysis of N-Acetylated and N-Deacetylated Derivatives of 4-Methylumbelliferyl Chitobioside by the Family 18 Chitinases ChiA and ChiB from Serratia Marcescens
- Substrate Specificity of the N, 6-ο-Diacetylmuramidase from Streptomyces globisporus
- An Investigation of the pH-Activity Relationships of Cex, a Family 10 Xylanase from Cellulomonas fimi : Xylan Inhibition and the Influence of Nitro-Substituted Aryl-β-D-Xylobiosides on Xylanase Activity
- グライコシンターゼ化による反転型加水分解酵素のグリコシド合成触媒への変換
- Prebiotic Effect of Lacto-N-biose I on Bifidobacterial Growth
- Characterization of Bacillus halodurans α-Galactosidase Me14A Encoded by the mel4A Gene (BH2228)
- Practical Preparation of D-Galactosyl-β1→4-L-rhamnose Employing the Combined Action of Phosphorylases
- Molecular Anatomy of the Alkaliphilic Xylanase from Bacillus halodurans C-125
- Employing Chimeric Xylanases to Identify Regions of an Alkaline Xylanase Participating in Fnzyme Activity at Basic pH(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Structural Explanation for the Acquisition of Glycosynthase Activity
- A Kinetic Study on pH-Activity Relationship of XynA from Alkaliphilic Bacillus halodurans C-125 Using Aryl-Xylobiosides
- Diversity and Similarity of Microbial Communities in Petroleum Crude Oils Produced in Asia
- Practical Preparation of Lacto-N-biose I, a Candidate for the Bifidus Factor in Human Milk
- Identification of the Putative Proton Donor Residue of Lacto-N-biose Phosphorylase (EC 2.4.1.211)
- Characterization of Glycosynthase Mutants Derived from Glycoside Hydrolase Family 10 Xylanases
- An Enzymatic Colorimetric Quantification of Orthophosphate
- Mutational Analysis of Fungal Family 11 Xylanases on pH Optimum Determination
- Interactions between Glycoside Hydrolase Family 94 Cellobiose Phosphorylase and Glucosidase Inhibitors
- p-Nitrophenyl β-Glycosides of β-1,4-Gluco/xylo-disaccharides for the Characterization of Subsites in Endo-xylanases
- Self-transferring Product Inhibition Observed during the Hydrolysis of Aryl-β-D-Glucopyranosides by a β-Glucosidase from Agrobacterium tumefaciens
- Characterization of a Bacterial Laminaribiose Phosphorylase