A Kinetic Study on pH-Activity Relationship of XynA from Alkaliphilic Bacillus halodurans C-125 Using Aryl-Xylobiosides

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概要

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• Xylanase A from alkaliphilic Bacillus halodurans C-125 was expressed in Eseherichia coli and purified by affinity and anion exchange chromatographies. It exhibited a strong substrate inhibition using xylan as the substrate. Its K, value increased with an increase in pH. The effect of pH on the enzyme activity was determined using two aryl-xylobiosides as substrates, and it was found that the enzyme had a flat k_cat-PH curve in the pH range of 5.8-8.8. This range was different from that obtained with 0.45% xylan as previously reported (Honda, H. et al., Agric. Biol. Chem., 49, 3165-3169, 1985). The substrate inhibition was presumed to cause the difference. It has been clarified that the use of aryl-xylobiosides as substrates yields more accurate kinetic results than that of xylan.

• 公益社団法人日本生物工学会の論文
• 2002-04-25

著者

• Kitaoka M

  Enzyme Laboratory National Food Research Institute

• Kitaoka M

  Chubu Univ. Aichi Jpn

• Miyatake K

  Osaka Prefecture Univ. Osaka Jpn

• Hayashi K

  Enzyme Laboratory National Food Research Institute

• HAYASHI Kiyoshi

  Enzyme Applications Laboratory, National Food Research Institute

• Kitaoka Motomitsu

  Enzyme Laboratory National Food Research Institute

• MATSUDA Kazuhiko

  Laboratory of Plant Pathology and Biotechnology and Laboratory of Pesticide Chemistry

• Kirihata Mitsunori

  Department Of Agricultural Chemistry Faculty Of Agriculture University Of Osaka Prefecture

• 切畑 光統

  大阪府立大学 農学部

• Kirihata Mitsunori

  Graduate School Of Life And Environmental Science Osaka Prefecture University

• Honda Yuji

  National Food Research Institute 2-1-12 Kannondai

• Honda Y

  Osaka Prefecture Univ. Sakai Jpn

• HONDA YUJI

  Enzyme Laboratory, National Food Research Institute

• Nishimoto Mamoru

  Enzyme Laboratory National Food Research Institute

• Honda Yuji

  Laboratory Of Bioorganic Chemistry College Of Agriculture Osaka Prefecture University

• Nishimoto Mamoru

  Enzyme Laboratory National Food Research Institute National Agriculture And Food Research Organizati

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