A Monitoring System for Green Fluorescence Protein Gene-transformed Fusarium oxysporum in Melon Seedlings
スポンサーリンク
概要
- 論文の詳細を見る
Using melon seedlings at the cotyledon stage and genetically marked fungi, a system for monitoring pathogenic and nonpathogenic Fusarium oxysporum was devised in the present study. Protoplasts were prepared from three formae speciales (melonis, radicis-lycopersici and fragariae) of F. oxysporum and transformed with a synthetic gene for green fluorescence protein. Transformants were primarily isolated in the presence of hygromycin B and then screened by the emission of bright green fluorescence. Roots of melon seedlings were inoculated with fluorescing microconidia of these fungi, and fungal infection behavior was traced. Using fluorescence microscopy, we directly observed not only the fungus at the root surface, but also the mycelia elongating in the trachea of roots. Both pathogenic and nonpathogenic fungi germinated and hyphae elongated superficially on the surface of root. Only pathogenilc fungi caused root necrosis at the inoculation site. Hyphae grew within the stem to induce constriction or cracking of lower hypocotyls, then causing wilting of the seedlings. Infection behavior of genetically marked pathogenic and nonpathogenic F. oxysporum could be successfully monitored after inoculation of cotyledons of seedlings.
- 日本植物病理学会の論文
- 2001-11-25
著者
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Miyatake K
Osaka Prefecture Univ. Osaka Jpn
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Takasugi Mikako
Laboratory of Food Science, Department of Food Science and Technology, Kyushu University School of A
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Matsuda Yoshinori
Laboratory Of Plant Pathology And Biotechnology Faculty Of Agriculture Kinki University
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Nonomura Teruo
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University
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Toyoda Hideyoshi
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University
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MATSUDA Kazuhiko
Laboratory of Plant Pathology and Biotechnology and Laboratory of Pesticide Chemistry
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Nonomura T
Laboratory Of Plant Pathology And Biotechnology Faculty Of Agriculture Kinki University
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Nonomura Teruo
Laboratory Of Plant Pathology And Biotechnology Faculty Of Agriculture Kinki University
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Kirihata Mitsunori
Department Of Agricultural Chemistry Faculty Of Agriculture University Of Osaka Prefecture
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切畑 光統
大阪府立大学 農学部
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Kirihata Mitsunori
Graduate School Of Life And Environmental Science Osaka Prefecture University
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Takasugi Mikako
Laboratory Of Plant Pathology And Biotechnology Faculty Of Agriculture Kinki University
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Toyoda Hideyoshi
Laboratory Of Plant Pathology And Biotechnology Faculty Of Agriculture Kinki University
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Hasegawa Tomoya
Laboratory Of Plant Pathology And Biotechnology Faculty Of Agriculture Kinki University
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Matsuda Yoshinori
Pharmaceutical Research And Technology Institute Kinki University
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OOTANI Takashi
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University
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MIYAJIMA Kazutomo
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University
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HATASA Tetsuya
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University
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Hatasa Tetsuya
Laboratory Of Plant Pathology And Biotechnology Faculty Of Agriculture Kinki University
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Ootani Takashi
Laboratory Of Plant Pathology And Biotechnology Faculty Of Agriculture Kinki University
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Matsuda Yoshinori
Institute For Comprehensive Agricultural Science Kinki University
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TAKASUGI Mikako
Laboratory of Food Science, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University
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