Detection of polymorphic patterns of genomic DNA amplified by RAPD-PCR in sweet potato weevils, Cylas formicarius (Fabricius) (Coleoptera : Brentidae)

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概要

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• The polymorphic patterns of genomic DNA amplified by RAPD-PCR were detected in laboratory-cultured populations of sweet potato weevils, Cylas formicarius collected from the Southwest islands (Nansei-Shoto) of Japan. When three sets of primers, T13/T13, T05/T05 and T01/T07 were used for PCR, the polymorphic patterns of the amplified DNA were classified into nine types. Of these types, A_1B_1C_3 was common in all populations tested. The present study determined the useful primer sets that will enable the successful classification of sweet potato weevils based upon the polymorphic patterns of genomic DNA amplified by RAPD-PCR.

• 日本応用動物昆虫学会の論文

著者

• Matsuda Yoshinori

  Laboratory Of Plant Pathology And Biotechnology Faculty Of Agriculture Kinki University

• Toyoda Hideyoshi

  Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University

• KAKUTANI Koji

  Pharmaceutical Research and Technology Institute, Kinki University

• Toyoda Hideyoshi

  Laboratory Of Plant Pathology And Biotechnology Faculty Of Agriculture Kinki University

• Matsuda Yoshinori

  Pharmaceutical Research And Technology Institute Kinki University

• SUGIMOTO Tuyosi

  Entomological Laboratory, Faculty of Agriculture, Kinki University

• Kawamura Kiyohisa

  Entomological Laboratory, Faculty of Agriculture, Kinki University

• Kawamura Kiyohisa

  Laboratory Of Ecological Entomology Faculty Of Agriculture Kinki University

• Sugimoto T

  Laboratory Of Ecological Entomology Faculty Of Agriculture Kinki University

• Sugimoto Tuyosi

  Entomological Laboratory Faculty Of Agriculture Kinki University

• Matsuda Yoshinori

  Institute For Comprehensive Agricultural Science Kinki University

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